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PMID: 6466316 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Volume-regulatory K+ efflux during concentrative uptake of alanine in isolated rat hepatocytes.

The Biochemical journal ·Vol. 221 ·No. 1 ·1984-07-01 ·Pages 265-8

Kristensen LO, Folke M

Abstract

Changes in cell volume and 42K+ efflux associated with concentrative alanine uptake were studied in isolated rat hepatocytes suspended in Krebs-Ringer bicarbonate buffer. After addition of 10 mM-alanine, cellular water volume increased by 15% and the rate constant of 42K+ efflux by 250%. Alanine-induced 42K+ efflux was abolished by quinine and was strongly decreased when the cell-volume increase was counteracted by sucrose. The results suggest that K+ efflux during alanine uptake is implicated in a volume-regulatory response.

MeSH Terms
Alanine/metabolism Animals Apamin/pharmacology Body Water/metabolism Female Furosemide/pharmacology In Vitro Techniques Liver/cytology,drug effects,metabolism Potassium/metabolism Quinine/pharmacology Rats Rats, Inbred Strains Sucrose/pharmacology
Chemicals
Apamin Sucrose Furosemide Quinine Alanine Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kristensen L O
Folke M
References (16)
16 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2210265
Published
1984-07-01
Pages
265-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144029
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2210265 PMC Full Text View journal details
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