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PMID: 6461856 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Quantitative model for gene regulation by lambda phage repressor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 4 ·1982-02-00 ·Pages 1129-33

Ackers GK, Johnson AD, Shea MA

Abstract

A statistical thermodynamic model has been developed to account for the cooperative interactions of the bacteriophage lambda repressor with the lambda right operator. The model incorporates a general theory for quantitatively interpreting cooperative site-specific equilibrium binding data. Values for all interaction parameters of the model have been evaluated at 37 degrees C, 0.2 M KCl, from results of DNase protection experiments in vitro [A. D. Johnson, B. J. Meyer, & M. Ptashne, Proc. Natl. Acad. Sci. USA (1979) 76, 5061-5065]. With these values, the model predicts repression curves at the divergent promoters PR and PRM that control transcription of genes coding for the regulatory proteins cro and repressor, respectively. At physiological repressor concentrations, repression at PR is predicted to be nearly complete whereas PRM is predicted to remain highly active. The results demonstrate the importance of cooperative interactions between repressor dimers bound to the adjacent operator sites OR1 and OR2 in maintaining a stable lysogenic state and in allowing efficient switchover to the lytic state during induction.

MeSH Terms

Bacteriophage lambda/genetics DNA-Binding Proteins Gene Expression Regulation Genes, Viral Lysogeny Mathematics Models, Genetic Operon Repressor Proteins/physiology Transcription Factors/physiology Viral Proteins Viral Regulatory and Accessory Proteins Virus Activation

Chemicals

DNA-Binding Proteins Repressor Proteins Transcription Factors Viral Proteins Viral Regulatory and Accessory Proteins phage repressor proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ackers G K

Johnson A D

Shea M A

References (23)

23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.4.1129

Published

1982-02-00

Pages

1129-33

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC345914

Subset

External Links

PubMed source DOI: 10.1073/pnas.79.4.1129 PMC Full Text View journal details

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