Home LiteratureArticle Details
PMID: 6430873 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Voltage clamp effects on bacterial chemotaxis.

Journal of bacteriology ·Vol. 159 ·No. 2 ·1984-08-00 ·Pages 605-10

Margolin Y, Eisenbach M

Abstract

To examine whether or not sensory signaling in bacteria is by way of fluctuations in membrane potential, we studied the effect of clamping the potential on bacterial chemotaxis. The potential was clamped by valinomycin, a K+ -specific ionophore, in the presence of K+. Despite the clamped potential, sensory signaling did occur: both Escherichia coli and Bacillus subtilis cells were still excitable and adaptable under these conditions. It is concluded that signaling in the excitation and adaptation steps of chemotaxis is not by way of fluctuations in the membrane potential.

MeSH Terms
Bacillus subtilis/drug effects,physiology Carbon Radioisotopes Chemotaxis/drug effects Escherichia coli/drug effects,physiology Membrane Potentials Nigericin/pharmacology Tritium Valinomycin/pharmacology
Chemicals
Carbon Radioisotopes Tritium Valinomycin Nigericin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Margolin Y
Eisenbach M
References (61)
61 references, click to expand
  1. pH homeostasis in Escherichia coli: measurement by 31P nuclear magnetic resonance of methylphosphonate and phosphate.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6271-5 PMID: 7031646
  2. The steady-state counterclockwise/clockwise ratio of bacterial flagellar motors is regulated by protonmotive force.
    J Mol Biol. 1980 Apr 15;138(3):563-97 PMID: 6774099
  3. Quantitative measurements of proton motive force and motility in Bacillus subtilis.
    J Bacteriol. 1980 Dec;144(3):891-7 PMID: 6254950
  4. In vivo and in vitro chemotactic methylation in Bacillus subtilis.
    J Bacteriol. 1981 Feb;145(2):958-65 PMID: 6780537
  5. Aerotaxis in Salmonella typhimurium: role of electron transport.
    J Bacteriol. 1981 Feb;145(2):990-1001 PMID: 6257658
  6. Change in intracellular pH of Escherichia coli mediates the chemotactic response to certain attractants and repellents.
    J Bacteriol. 1981 Mar;145(3):1196-208 PMID: 7009571
  7. Cytoplasmic pH mediates pH taxis and weak-acid repellent taxis of bacteria.
    J Bacteriol. 1981 Mar;145(3):1209-21 PMID: 7009572
  8. Kinetic properties of ion carriers and channels.
    J Membr Biol. 1980 Dec 30;57(3):163-78(-RETURN-) PMID: 6162960
  9. Bacterial cell envelopes with functional flagella.
    J Biol Chem. 1981 Aug 25;256(16):8807-14 PMID: 7021551
  10. Chemotaxis of Spirochaeta aurantia: involvement of membrane potential in chemosensory signal transduction.
    J Bacteriol. 1981 Dec;148(3):837-44 PMID: 7309678
  11. Measurement of membrane potential in Bacillus subtilis: a comparison of lipophilic cations, rubidium ion, and a cyanine dye as probes.
    J Membr Biol. 1981;63(3):215-31 PMID: 6796695
  12. Role of membrane potential and calcium in chemotactic sensing by bacteria.
    J Mol Biol. 1981 Jun 25;149(2):241-57 PMID: 6796698
  13. Signal processing times in bacterial chemotaxis.
    Nature. 1982 Apr 29;296(5860):855-7 PMID: 7040985
  14. Quantitative measurements of the proton-motive force and its relation to steady state lactose accumulation in Escherichia coli.
    Biochem J. 1981 Dec 15;200(3):573-81 PMID: 6282253
  15. Membrane potential in a potassium transport-negative mutant of Escherichia coli K-12. The distribution of rubidium in the presence of valinomycin indicates a higher potential than that of the tetraphenylphosphonium cation.
    Biochim Biophys Acta. 1982 Sep 15;681(3):474-83 PMID: 6812627
  16. A voltage clamp inhibits chemotaxis of Spirochaeta aurantia.
    J Bacteriol. 1983 Feb;153(2):916-20 PMID: 6822479
  17. Changes in membrane potential of Escherichia coli in response to temporal gradients of chemicals.
    Biochemistry. 1982 Dec 21;21(26):6818-25 PMID: 6760894
  18. Correlation between bacteriophage chi adsorption and mode of flagellar rotation of Escherichia coli chemotaxis mutants.
    J Bacteriol. 1983 May;154(2):604-11 PMID: 6341356
  19. Coordination of flagella on filamentous cells of Escherichia coli.
    J Bacteriol. 1983 Jul;155(1):228-37 PMID: 6345503
  20. Dynamics and energetics of flagellar rotation in bacteria.
    Symp Soc Exp Biol. 1982;35:1-31 PMID: 6306855
  21. The use of valinomycin, nigericin and trichlorocarbanilide in control of the protonmotive force in Escherichia coli cells.
    Biochem J. 1983 Apr 15;212(1):105-12 PMID: 6307285
  22. Sensory transduction in bacterial chemotaxis.
    Int Rev Cytol. 1983;81:33-70 PMID: 6307914
  23. A process related to membrane potential involved in bacterial chemotaxis to galactose.
    Biochemistry. 1983 Jun 21;22(13):3293-8 PMID: 6349685
  24. Inhibition of Spirochaeta aurantia chemotaxis by neurotoxins.
    J Bacteriol. 1983 Sep;155(3):1443-5 PMID: 6136501
  25. Role of proton motive force in sensory transduction in bacteria.
    Annu Rev Microbiol. 1983;37:551-73 PMID: 6314878
  26. Distinction between changes in membrane potential and surface charge upon chemotactic stimulation of Escherichia coli.
    Biophys J. 1984 Feb;45(2):463-7 PMID: 6365190
  27. Direction of flagellar rotation in bacterial cell envelopes.
    J Bacteriol. 1984 Apr;158(1):222-30 PMID: 6370958
  28. Minimal requirements for rotation of bacterial flagella.
    J Bacteriol. 1984 Jun;158(3):1208-10 PMID: 6373740
  29. Release of lipopolysaccharide by EDTA treatment of E. coli.
    Biochem Biophys Res Commun. 1965 Nov 22;21(4):290-6 PMID: 4159978
  30. Nonchemotactic mutants of Escherichia coli.
    J Bacteriol. 1967 Jan;93(1):390-8 PMID: 5335897
  31. Inhibitio of flagellar coordination in Spirillum volutans.
    J Bacteriol. 1967 Nov;94(5):1431-6 PMID: 6057800
  32. Studies on the permeability change produced in coliform bacteria by ethylenediaminetetraacetate.
    J Biol Chem. 1968 May 10;243(9):2373-80 PMID: 4967584
  33. Effect of drugs that alter excitable membranes on the motility of Rhodospirillum rubrum and Thiospirillum jenense.
    Can J Microbiol. 1971 Feb;17(2):191-6 PMID: 4251321
  34. The gradient-sensing mechanism in bacterial chemotaxis.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2509-12 PMID: 4560688
  35. Chemotaxis toward amino acids in Escherichia coli.
    J Bacteriol. 1972 Oct;112(1):315-26 PMID: 4562400
  36. Uncoordination and recoordination in Spirillum volutans.
    Can J Microbiol. 1972 Nov;18(11):1749-59 PMID: 5086110
  37. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli.
    J Gen Microbiol. 1973 Jan;74(1):77-91 PMID: 4632978
  38. Negative chemotaxis in Escherichia coli.
    J Bacteriol. 1974 May;118(2):560-76 PMID: 4597449
  39. Change in direction of flagellar rotation is the basis of the chemotactic response in Escherichia coli.
    Nature. 1974 May 3;249(452):74-7 PMID: 4598031
  40. Chemomechanical coupling without ATP: the source of energy for motility and chemotaxis in bacteria.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1239-43 PMID: 4598295
  41. The requirement for energy transducing ATPase for anaerobic motility in Escherichia coli.
    Biochim Biophys Acta. 1974 Jun 28;347(3):464-8 PMID: 4276403
  42. Chemotaxis in bacteria.
    Annu Rev Biophys Bioeng. 1975;4(00):119-36 PMID: 1098551
  43. The proton electrochemical gradient in Escherichia coli cells.
    Eur J Biochem. 1976 Apr 1;63(2):533-41 PMID: 4325
  44. Motility in normal and filamentous forms of Rhodospirillum rubrum.
    J Gen Microbiol. 1976 Apr;93(2):346-54 PMID: 819618
  45. Chemotaxis toward amino acids by Bacillus subtilis.
    J Bacteriol. 1977 Jan;129(1):151-5 PMID: 12134
  46. Change in membrane potential during bacterial chemotaxis.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4387-91 PMID: 794876
  47. Proton-motive force and the motile behavior of Bacillus subtilis.
    Arch Microbiol. 1976 Dec 1;111(1-2):7-11 PMID: 13758
  48. The electrochemical proton gradient in Escherichia coli membrane vesicles.
    Biochemistry. 1977 Mar 8;16(5):848-54 PMID: 14664
  49. Phototaxis and membrane potential in the photosynthetic bacterium Rhodospirillum rubrum.
    J Bacteriol. 1977 Jul;131(1):34-41 PMID: 194880
  50. A protonmotive force drives bacterial flagella.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):3060-4 PMID: 19741
  51. Sensory electrophysiology of bacteria: relationship of the membrane potential to motility and chemotaxis in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4752-6 PMID: 412194
  52. Control of the chemotactic behavior of Bacillus subtilis cells.
    Arch Microbiol. 1978 Jan 23;116(1):1-8 PMID: 23735
  53. The proton pump is a molecular engine of motile bacteria.
    Nature. 1978 Mar 16;272(5650):280-2 PMID: 24186
  54. Failure of sensory adaptation in bacterial mutants that are defective in a protein methylation reaction.
    Cell. 1978 Dec;15(4):1231-40 PMID: 365357
  55. Ionophores.
    Methods Enzymol. 1979;55:435-54 PMID: 379502
  56. The measurement of membrane potential and deltapH in cells, organelles, and vesicles.
    Methods Enzymol. 1979;55:547-69 PMID: 37402
  57. Protein methylation in behavioural control mechanisms and in signal transduction.
    Nature. 1979 Jul 26;280(5720):279-84 PMID: 379649
  58. Characterization of the Bacillus subtilis motile system driven by an artificially created proton motive force.
    J Bacteriol. 1979 Oct;140(1):28-36 PMID: 40954
  59. Protonmotive force and bacterial sensing.
    J Bacteriol. 1980 Jan;141(1):26-32 PMID: 6766440
  60. Energetics of flagellar rotation in bacteria.
    J Mol Biol. 1980 Apr 15;138(3):541-61 PMID: 7411617
  61. Proton chemical potential, proton electrical potential and bacterial motility.
    J Mol Biol. 1980 Apr 15;138(3):599-614 PMID: 6774100
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.159.2.605-610.1984
Published
1984-08-00
Pages
605-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215686
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.159.2.605-610.1984 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2