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PMID: 6426470 Published · ppublish English Journal Article

Isolation and characterization of a novel 68,000-Mr Ca2+-binding protein of lymphocyte plasma membrane.

The Biochemical journal ·Vol. 219 ·No. 1 ·1984-04-01 ·Pages 309-16

Owens RJ, Crumpton MJ

Abstract

A 68 000-Mr protein is a major component of a Nonidet P-40-insoluble fraction of lymphocyte plasma membrane prepared from human B lymphoblastoid cells ( BRI 8) and pig mesenteric lymph nodes. The association of the protein with the detergent-insoluble complex depends on free Ca2+ concentrations of greater than 10 microM. The human and pig 68 000-Mr proteins were purified and appear to be homologous on the basis of amino acid composition and peptide mapping. The protein is monomeric, has pI 5.8 and a single high-affinity Ca2+-binding site (KD 1.2 microM). The results are discussed in terms of the possible role of the 68 000-Mr protein as an intracellular Ca2+ receptor in lymphocytes.

MeSH Terms
Amino Acids/analysis Animals Annexin A6 Calcium-Binding Proteins/isolation & purification Cell Line Cell Membrane/analysis Chromatography, Gel Egtazic Acid Electrophoresis, Polyacrylamide Gel Humans Lymphocytes/analysis Membrane Proteins/isolation & purification Molecular Weight Octoxynol Polyethylene Glycols Swine
Chemicals
Amino Acids Annexin A6 Calcium-Binding Proteins Membrane Proteins Polyethylene Glycols Egtazic Acid Octoxynol Nonidet P-40
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Owens R J
Crumpton M J
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2190309
Published
1984-04-01
Pages
309-16
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1153478
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2190309 PMC Full Text View journal details
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