Home LiteratureArticle Details
PMID: 6423932 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Revised locations of the hisI and pru (proline utilization) genes on the Pseudomonas aeruginosa chromosome map.

Molecular & general genetics : MGG ·Vol. 193 ·No. 3 ·1984-00-00 ·Pages 431-6

Soldati L, Crockett R, Carrigan JM, Leisinger T, Holloway BW, Haas D

Abstract

The location of genes in the vicinity of the major FP2 origin on the chromosome of Pseudomonas aeruginosa PAO has been revised. The markers hisI (a transduction group of histidine biosynthetic genes) and pru (a gene cluster encoding proline utilization functions) were located in the 90 to 95/0 min chromosome region by a series of plate matings mediated by R68.45. Three-factor-crosses using this plasmid established the following marker order: pur-67 pru hisI/cys-59 proB ilvB/C. Genetic evidence is presented to confirm the previous observations that FP2 can mobilize the chromosome from at least two origins near proB and in both directions. Thus, when markers in this chromosome region are analyzed by FP2 crosses only, the mapping data may be difficult to interpret. This complication can be overcome by the use of R68.45 and Tfr (transposon-facilitated recombination) or Hfr donors.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial/physiology Crosses, Genetic Genes, Bacterial Genetic Linkage Genotype Histidine/genetics Plasmids Proline/genetics Pseudomonas aeruginosa/genetics Transduction, Genetic
Chemicals
Histidine Proline
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Soldati L
Crockett R
Carrigan J M
Leisinger T
Holloway B W
Haas D
References (34)
34 references, click to expand
  1. ABSENCE OF CLUSTERING OF FUNCTIONALLY RELATED GENES IN PSEUDOMONAS AERUGINOSA.
    Genet Res. 1965 Jul;6:284-99 PMID: 14345912
  2. A novel transducing phage. Its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa.
    Mol Gen Genet. 1972;114(2):134-43 PMID: 4622961
  3. Chromosome mobilization by the R plasmid R68.45: a tool in Pseudomonas genetics.
    Mol Gen Genet. 1978 Jan 17;158(3):229-37 PMID: 415223
  4. Regulation of the genes for proline utilization in Salmonella typhimurium: autogenous repression by the putA gene product.
    J Mol Biol. 1981 May 5;148(1):21-44 PMID: 7031262
  5. Genetic recombination in Pseudomonas aeruginosa.
    J Gen Microbiol. 1955 Dec;13(3):572-81 PMID: 13278508
  6. Biosynthesis of proline in Pseudomonas aeruginosa. Properties of gamma-glutamyl phosphate reductase and 1-pyrroline-5-carboxylate reductase.
    Biochem J. 1979 Jul 1;181(1):223-30 PMID: 114173
  7. Genetic circularity of the Pseudomonas aeruginosa PAO chromosome.
    J Bacteriol. 1981 Jan;145(1):145-55 PMID: 6780510
  8. Isolation of an Hfr donor of Pseudomonas aeruginosa PAO by insertion of the plasmid RP1 into the tryptophan synthase gene.
    Mol Gen Genet. 1981;182(2):240-4 PMID: 6270503
  9. A map order for his I, one of the genetic regions controlling histidine biosynthesis in Pseudomonas aeruginosa, using the transducing phage F116.
    Genetics. 1969 Jul;62(3):687-96 PMID: 4988584
  10. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  11. Uniform nomenclature for bacterial plasmids: a proposal.
    Bacteriol Rev. 1976 Mar;40(1):168-89 PMID: 1267736
  12. Control of pyrimidine biosynthesis in Pseudomonas aeruginosa.
    J Bacteriol. 1968 Nov;96(5):1732-41 PMID: 4973129
  13. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
  14. The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa.
    Mol Gen Genet. 1977 Jul 7;154(1):7-22 PMID: 408599
  15. An analysis of histidine requiring mutants in Pseudomonas aeruginosa.
    Genetics. 1967 Apr;55(4):709-22 PMID: 4962509
  16. A mutant of Pseudomonas aeruginosa deficient in an ATP-dependent deoxyribonuclease.
    J Gen Microbiol. 1980 Oct;120(2):377-84 PMID: 6785384
  17. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa.
    Mol Gen Genet. 1976 Mar 30;144(3):243-51 PMID: 818504
  18. Chromosome mapping in Pseudomonas aeruginosa.
    Genet Res. 1972 Jun;19(3):251-60 PMID: 4627640
  19. Purification and properties of the bifunctional proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase from Pseudomonas aeruginosa.
    Eur J Biochem. 1982 Dec;129(1):67-75 PMID: 6819140
  20. A method for the selection of deletion mutations in the L-proline catabolism gene cluster of Aspergillus nidulans.
    Genet Res. 1981 Oct;38(2):171-95 PMID: 7035292
  21. The order of replication of chromosomal markers in Pseudomonas aeruginosa strain 1. I. Marker frequency analysis by transduction.
    Genet Res. 1974 Apr;23(2):145-53 PMID: 4214214
  22. Investigation of the mating system of Pseudomonas aeruginosa strain 1. IV. Mapping of distal markers.
    Genet Res. 1969 Feb;13(1):91-8 PMID: 4977062
  23. Conjugation in Pseudomonas aeruginosa.
    Genetics. 1969 Feb;61(2):327-39 PMID: 4980017
  24. Investigation of the mating system of Pseudomonas aeruginosa strain 1. II. Mapping of a number of early markers.
    Genet Res. 1968 Aug;12(1):37-44 PMID: 4976997
  25. Chromosomal location of genes participating in the degradation of purines in Pseudomonas aeruginosa.
    Mol Gen Genet. 1978 Nov 29;167(2):165-76 PMID: 104142
  26. Chromosomal genetics of Pseudomonas.
    Microbiol Rev. 1979 Mar;43(1):73-102 PMID: 111024
  27. Alginate synthesis in mucoid Pseudomonas aeruginosa: a chromosomal locus involved in control.
    J Gen Microbiol. 1980 Aug;119(2):443-50 PMID: 6785378
  28. Regulation of proline catabolism in Pseudomonas aeruginosa PAO.
    Arch Microbiol. 1982 Aug;132(2):189-93 PMID: 6812528
  29. Size of the chromosome of Pseudomonas aeruginosa PAO.
    J Bacteriol. 1974 Sep;119(3):748-52 PMID: 4212339
  30. Isolation and properties of recombination-deficient mutants of Pseudomonas aeruginosa.
    Mutat Res. 1974 Apr;23(1):15-23 PMID: 4209312
  31. New prime plasmids from Pseudomonas aeruginosa.
    Genet Res. 1981 Jun;37(3):265-74 PMID: 6790345
  32. Indospicine as an arginine antagonist in Escherichia coli and Pseudomonas aeruginosa.
    Biochim Biophys Acta. 1972 Mar 14;262(2):214-9 PMID: 4336093
  33. Insertions of the transposon Tn1 into the Pseudomonas aeruginosa chromosome.
    Genetics. 1981 Mar-Apr;97(3-4):495-511 PMID: 6271621
  34. Control of arginine biosynthesis in Pseudomonas aeruginosa.
    J Gen Microbiol. 1972 Dec;73(3):427-38 PMID: 4632763
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00382080
Published
1984-00-00
Pages
431-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00382080
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2