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PMID: 6421982 Published · ppublish English Journal Article

Biological and antigenic similarities of murine interferon-gamma and macrophage-activating factor.

The Journal of experimental medicine ·Vol. 159 ·No. 3 ·1984-03-01 ·Pages 812-27

Svedersky LP, Benton CV, Berger WH, Rinderknecht E, Harkins RN, Palladino MA

Abstract

Murine peritoneal exudate cells (PEC) treated with murine recombinant interferon-gamma (IFN-gamma) (greater than 99% estimated purity), or concanavalin A-stimulated spleen cell supernatants developed tumoricidal properties (macrophage activation factor [MAF] activity). MAF activity was found to occur with treatments of 10 U/ml IFN-gamma, and at levels as low as 1 U/ml IFN-gamma if a second signal (5 ng/ml endotoxin) was present in the MAF assay. Endotoxin (lipopolysaccharide [LPS]) alone at these levels failed to induce MAF; induction of MAF was observed at 1,000-fold greater levels. The ability of IFN-gamma to stimulate murine PEC was species specific. Various sources of materials that displayed MAF activity, including supernatants from interleukin 2-dependent cloned cytotoxic murine T lymphocyte lines that did not display detectable antiviral activity, were neutralized by antibody raised and affinity purified against recombinant IFN-gamma. Thus, IFN-gamma, although never detectable by antiviral assays, appears to be present in many lymphokine preparations and has potent macrophage activation capability.

MeSH Terms
Animals Ascitic Fluid/immunology Cell Line Cytotoxicity, Immunologic Female Guinea Pigs Humans Interferon-gamma/biosynthesis,immunology,physiology Kinetics Lymphocyte Activation Lymphokines/biosynthesis,immunology,physiology Macrophage Activation Macrophage-Activating Factors Male Mice Mice, Inbred C57BL Neutralization Tests Rabbits T-Lymphocytes/immunology
Chemicals
Lymphokines Macrophage-Activating Factors Interferon-gamma
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Svedersky L P
Benton C V
Berger W H
Rinderknecht E
Harkins R N
Palladino M A
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43 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.159.3.812
Published
1984-03-01
Pages
812-27
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187242
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.159.3.812 PMC Full Text View journal details
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