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PMID: 6407020 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Macrophage activation: priming activity from a T-cell hybridoma is attributable to interferon-gamma.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 12 ·1983-06-00 ·Pages 3782-6

Pace JL, Russell SW, Schreiber RD, Altman A, Katz DH

Abstract

Antiviral and macrophage-priming activities in the supernatant medium of a subclone of a concanavalin A-stimulated mouse T-cell hybridoma were investigated. The two activities were associated with a molecular weight of approximately 50,000 and could not be separated by various approaches. Both activities were eliminated by a highly specific neutralizing antibody against mouse interferon-gamma, but not by antibody against interferon-alpha and -beta. The ratio of priming to antiviral activity in the hybridoma culture supernate was indistinguishable from the ratio obtained with mouse interferon-gamma prepared by recombinant DNA technology. It was concluded from these data that the priming activity in hybridoma culture supernates was attributable to interferon-gamma and that this mediator is one form of the lymphokine macrophage-activating factor. Interferon-gamma was greater than 800 times more efficient at priming mouse macrophages for tumor cell killing than was a mixture of interferon-alpha and -beta. This finding contributes to growing awareness that type II interferon may have greater immunoregulatory potential than type I interferons.

MeSH Terms
Animals Antibodies Cells, Cultured Concanavalin A Hybridomas/immunology Interferon Type I/immunology Interferon-gamma/immunology Macrophage Activation Macrophages/immunology Male Mice Mice, Inbred Strains T-Lymphocytes/immunology
Chemicals
Antibodies Interferon Type I Concanavalin A Interferon-gamma
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pace J L
Russell S W
Schreiber R D
Altman A
Katz D H
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.12.3782
Published
1983-06-00
Pages
3782-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394136
Subset
IM
Grants
NCI NIH HHS · CA 25803 · United States
NCI NIH HHS · CA 31199 · United States
NCI NIH HHS · CA 34120 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.12.3782 PMC Full Text View journal details
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