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PMID: 6417659 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the human receptor for T-cell growth factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 22 ·1983-11-00 ·Pages 6957-61

Leonard WJ, Depper JM, Robb RJ, Waldmann TA, Greene WC

Abstract

Anti-Tac monoclonal antibody has been identified as a putative antibody against the receptor for T-cell growth factor (TCGF). We now show that: (i) TCGF blocks 85% of 3H-labeled anti-Tac binding to phytohemagglutinin-activated lymphoblasts and (ii) both anti-Tac and anti-TCGF immunoprecipitate a protein band that appears to represent TCGF crosslinked to its receptor on HUT-102B2 cells. In HUT-102B2 cells, the TCGF receptor is a Mr 50,000 glycoprotein with internal disulfide bond(s) and a pI of 5.5-6.0, and it represents approximately equal to 0.05% of total cellular de novo protein synthesis. It contains a peptide of Mr 33,000 that is processed to a mature form that includes N-linked and O-linked sugars and sialic acid.

MeSH Terms
Cell Line Glycoside Hydrolases/pharmacology Humans Interleukin-2/immunology Kinetics Lymphoma/immunology Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Molecular Weight Neuraminidase/pharmacology Receptors, Immunologic/immunology,isolation & purification Receptors, Interleukin-2 T-Lymphocytes/drug effects,immunology Tunicamycin/pharmacology
Chemicals
Interleukin-2 Receptors, Immunologic Receptors, Interleukin-2 Tunicamycin Glycoside Hydrolases Neuraminidase Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Leonard W J
Depper J M
Robb R J
Waldmann T A
Greene W C
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.22.6957
Published
1983-11-00
Pages
6957-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390105
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.22.6957 PMC Full Text View journal details
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