Purification and characterization of the IIIXtl phospho-carrier protein of the phosphoenolpyruvate-dependent xylitol:phosphotransferase found in Lactobacillus casei C183.

Journal of bacteriology ·Vol. 156 ·No. 2 ·1983-11-00 ·Pages 611-9

London J, Hausman SZ

Abstract

The phosphoenolpyruvate-dependent xylitol:phosphotransferase system of Lactobacillus casei strain C183 requires a small, soluble, substrate-specific protein for catalytic activity. Designated enzyme IIIXtl (or IIIXtl), the protein was purified to electrophoretic homogeneity and characterized. IIIXtl, as purified, is a single polypeptide composed of 109 amino acid residues. It has an estimated molecular weight of 12,000 and is hydrophobic in nature. The hydrophobicity of IIIXtl is apparently due to the fact that the enzyme was isolated as the phosphorylated phosphocarrier protein. Removal of the phosphate group with alkaline phosphatase results in the loss of immunological cross-reactivity with anti-P-IIIXtl and an alteration in charge. The L. casei C183 IIIXtl is antigenically related to enzymes IIIXtl in Streptococcus avium and other, genetically distinct strains of L. casei.

MeSH Terms

Amino Acids/analysis Bacterial Proteins Electrophoresis, Polyacrylamide Gel Fatty Acids/analysis Immunodiffusion Lactobacillus casei/enzymology Molecular Weight Phosphoenolpyruvate/metabolism Phosphoenolpyruvate Sugar Phosphotransferase System/isolation & purification,metabolism

Chemicals

Amino Acids Bacterial Proteins Fatty Acids Phosphoenolpyruvate Phosphoenolpyruvate Sugar Phosphotransferase System phosphoenolpyruvate-xylitol phosphotransferase, Lactobacillus casei

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

London J

Hausman S Z

References (23)

23 references, click to expand

Sugar transport. V. A trimeric lactose-specific phosphocarrier protein of the Staphylococcus aureus phosphotransferase system.
J Biol Chem. 1973 Feb 10;248(3):941-56 PMID: 4684715
New pathway for the metabolism of pentitols.
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4296-300 PMID: 16592445
Regulation of lactose-phosphoenolpyruvate-dependent phosphotransferase system and beta-D-phosphogalactoside galactohydrolase activities in Lactobacillus casei.
J Bacteriol. 1983 Jun;154(3):1195-203 PMID: 6406426
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
Xylitol-mediated transient inhibition of ribitol utilization by Lactobacillus casei.
J Bacteriol. 1982 May;150(2):657-61 PMID: 6802797
Pentitol metabolism in Lactobacillus casei.
J Bacteriol. 1979 Dec;140(3):949-54 PMID: 118163
Estimation of the molecular weights of proteins by Sephadex gel-filtration.
Biochem J. 1964 May;91(2):222-33 PMID: 4158310
Phosphoenolpyruvate-dependent phosphotransferase system of Staphylococcus aureus: factor IIIlac, a trimeric phospho-carrier protein that also acts as a phase transfer catalyst.
Biochemistry. 1982 Sep 28;21(20):4867-73 PMID: 7138836
A modified procedure for the automatic analysis of amino acids.
Anal Biochem. 1960 Nov;1:187-201 PMID: 13735644
Immunological studies with genetically altered beta-galactosidases.
Ann N Y Acad Sci. 1963 May 8;103:1058-66 PMID: 13942583
Regulation and characterization of the galactose-phosphoenolpyruvate-dependent phosphotransferase system in Lactobacillus casei.
J Bacteriol. 1983 Jun;154(3):1204-14 PMID: 6406427
Sugar transport by the bacterial phosphotransferase system. Isolation and characterization of a glucose-specific phosphocarrier protein (IIIGlc) from Salmonella typhimurium.
J Biol Chem. 1982 Dec 10;257(23):14526-37 PMID: 6754734
In vivo regulation of glycolysis and characterization of sugar: phosphotransferase systems in Streptococcus lactis.
J Bacteriol. 1978 Nov;136(2):465-76 PMID: 101523
Isolation of IIIGlc of the phosphoenolpyruvate-dependent glucose phosphotransferase system of Salmonella typhimurium.
J Bacteriol. 1981 Oct;148(1):257-64 PMID: 7026533
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Aldolase of lactic acid bacteria: a case history in the use of an enzyme as an evolutionary marker.
Bacteriol Rev. 1973 Dec;37(4):453-78 PMID: 4203394
The bacterial PEP-dependent phosphotransferase system mechanism of gluconate phosphorylation in Streptococcus faecalis.
FEBS Lett. 1982 Feb 8;138(1):101-3 PMID: 6802670
Catabolism of unnatural carbohydrates by micro-organisms.
Adv Microb Physiol. 1976;13:1-53 PMID: 775941
Polypeptides of the tail fibres of bacteriophage T4.
J Mol Biol. 1971 Dec 28;62(3):465-77 PMID: 5136579
Group Q streptococci. I. Ecology, serology, physiology, and relationship to established enterococci.
J Bacteriol. 1967 Aug;94(2):291-6 PMID: 4962699
Serum antibodies that distinguish between the phospho- and dephospho-forms of a phosphoprotein.
Nature. 1982 Oct 21;299(5885):734-6 PMID: 6289133
Possible evolutionary significance of polyunsaturated fatty acids in blue-green algae.
Nature. 1970 Sep 12;227(5263):1164-6 PMID: 4988959

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.156.2.611-619.1983

Published

1983-11-00

Pages

611-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC217874

Subset

External Links

PubMed source DOI: 10.1128/jb.156.2.611-619.1983 PMC Full Text View journal details

Analysis Services

Purification and characterization of the IIIXtl phospho-carrier protein of the phosphoenolpyruvate-dependent xylitol:phosphotransferase found in Lactobacillus casei C183.

Contact

WeChat Official Account