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PMID: 6406427 Published · ppublish English Journal Article

Regulation and characterization of the galactose-phosphoenolpyruvate-dependent phosphotransferase system in Lactobacillus casei.

Journal of bacteriology ·Vol. 154 ·No. 3 ·1983-06-00 ·Pages 1204-14

Chassy BM, Thompson J

Abstract

Cells of Lactobacillus casei grown in media containing galactose or a metabolizable beta-galactoside (lactose, lactulose, or arabinosyl-beta-D-galactoside) were induced for a galactose-phosphoenolpyruvate-dependent phosphotransferase system (gal-PTS). This high-affinity system (Km for galactose, 11 microM) was inducible in eight strains examined, which were representative of all five subspecies of L. casei. The gal-PTS was also induced in strains defective in glucose- and lactose-phosphoenolpyruvate-dependent phosphotransferase systems during growth on galactose. Galactose 6-phosphate appeared to be the intracellular inducer of the gal-PTS. The gal-PTS was quite specific for D-galactose, and neither glucose, lactose, nor a variety of structural analogs of galactose caused significant inhibition of phosphotransferase system-mediated galactose transport in intact cells. The phosphoenolpyruvate-dependent phosphorylation of galactose in vitro required specific membrane and cytoplasmic components (including enzyme IIIgal), which were induced only by growth of the cells on galactose or beta-galactosides. Extracts prepared from such cells also contained an ATP-dependent galactokinase which converted galactose to galactose 1-phosphate. Our results demonstrate the separate identities of the gal-PTS and the lactose-phosphoenol-pyruvate-dependent phosphotransferase system in L. casei.

MeSH Terms
Adenosine Triphosphate/pharmacology Enzyme Induction Galactokinase/metabolism Galactose/metabolism Galactosephosphates/metabolism Lactobacillus casei/enzymology Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism Phosphorylation Substrate Specificity
Chemicals
Galactosephosphates galactose-1-phosphate galactose-6-phosphate Adenosine Triphosphate Phosphoenolpyruvate Sugar Phosphotransferase System phosphoenolpyruvate-galactose phosphotransferase Galactokinase Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chassy B M
Thompson J
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30 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.154.3.1204-1214.1983
Published
1983-06-00
Pages
1204-14
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217592
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.154.3.1204-1214.1983 PMC Full Text View journal details
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