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PMID: 6402489 Published · ppublish English Journal Article

Genetic mapping of bra genes affecting branched-chain amino acid transport in Pseudomonas aeruginosa.

Journal of bacteriology ·Vol. 153 ·No. 3 ·1983-03-00 ·Pages 1272-81

Hoshino T, Tsuda M, Iino T, Nishio K, Kageyama M

Abstract

Pseudomonas aeruginosa PAO mutants defective in the transport systems for branched-chain amino acids were isolated and characterized. Two mutations in strains selected for trifluoroleucine resistance, braA300 and braB307, were mapped in the met-9020-dcu-9108 and the nar-9011-puuC10 region, respectively. The mutation loci in strains selected for azaleucine resistance, braC310 and bra-311 through bra-314, were all located near the fla genes, with an order of region I fla-bra-region II fla. Strains with braA300 showed a marked reduction in the high-affinity branched-chain amino acid transport system (LIV-I) and a considerable decrease in the lower-affinity system (LIV-II). Strains with braB307 were found to be defective in the LIV-II system. Strains selected for azaleucine resistance were all defective only in the LIV-I system and fell into three phenotypically distinct classes. Strains with braC310 produced a binding protein for leucine, isoleucine, valine, alanine, and threonine (LIVAT-BP) altered in binding ability, indicating that the braC gene is the structural one for the LIVAT-BP. Strains with bra-311 or bra-312 showed a complete loss of production of the LIVAT-BP. Strains with bra-313 or bra-314 produced normal levels of functional LIVAT-BP, suggesting that these mutations are located in a gene(s) other than braC.

MeSH Terms
Amino Acids, Branched-Chain/metabolism Biological Transport, Active Chromosome Mapping Electrophoresis, Polyacrylamide Gel Genes, Bacterial Mutation Phenotype Pseudomonas aeruginosa/genetics
Chemicals
Amino Acids, Branched-Chain
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hoshino T
Tsuda M
Iino T
Nishio K
Kageyama M
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.153.3.1272-1281.1983
Published
1983-03-00
Pages
1272-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221773
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.153.3.1272-1281.1983 PMC Full Text View journal details
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