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PMID: 6401701 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transductional analysis of the flagellar genes in Pseudomonas aeruginosa.

Journal of bacteriology ·Vol. 153 ·No. 2 ·1983-02-00 ·Pages 1018-26

Tsuda M, Iino T

Abstract

Complementation in bacteriophage E79 tv-l-mediated transduction and the phenotypic properties of the flagellar genes in Pseudomonas aeruginosa PAO were investigated by using 195 flagellar mutants of this organism. A total of 15 fla. 1 mot, and 2 che cistrons were identified. At least 5 fla cistrons (fla V to flaZ) and one mot cistron resided in one region, and at least 10 fla cistrons (flaA to flaJ) and two che cistrons (cheA and cheB) resided in another. The flaC mutants exhibited cistron-specific leakiness on motility agar plates. The flaE cistron may be the structural gene for the component protein of the flagellar filament. The cheA mutations, which resulted in pleiotropic phenotypes for flagellar formation, motility, and taxis, belonged to the same complementation group as the flaF mutations; that is, we inferred that cheA and flaF are synonymous.

MeSH Terms
Flagella Genes Genes, Bacterial Genetic Complementation Test Mutation Phenotype Pseudomonas aeruginosa/genetics,ultrastructure Transduction, Genetic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tsuda M
Iino T
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.153.2.1018-1026.1983
Published
1983-02-00
Pages
1018-26
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221726
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.153.2.1018-1026.1983 PMC Full Text View journal details
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