Home LiteratureArticle Details
PMID: 6392823 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mapping of markers in the gyrA-his region of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 197 ·No. 1 ·1984-00-00 ·Pages 175-81

Middendorf A, Schweizer H, Vreemann J, Boos W

Abstract

Discrepancies between our mapping data concerning the cdd and gat marker of Escherichia coli and data obtained by Josephsen et al. (1983) as well as with the current linkage map of Escherichia coli (Bachmann 1983) led us to reinvestigate the mapping of markers in the gyrA-his region by P1 transduction. In addition, we isolated Hfr strains by integrating the temperature-sensitive F'ts114lac+ episome via lac homology of cir'lacZ and mgl'lacZ fusions into the chromosome. From the results of the P1 transductions and using these Hfr strains as donors in crosses it became clear that the transcription of cir and mgl is the same and counterclockwise on the chromosome, with cdd and gat as the counterclockwise markers to mgl and cir. We conclude that our previously published sequence of markers was incorrect and is in fact inverted. The present data indicate the following sequence of markers in clockwise order: his gat cdd mgl cir fpk gyrA.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Escherichia coli/genetics Genes, Bacterial Genetic Markers Transcription, Genetic
Chemicals
Genetic Markers
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Middendorf A
Schweizer H
Vreemann J
Boos W
References (25)
25 references, click to expand
  1. Mutations affecting transport of the hexitols D-mannitol, D-glucitol, and galactitol in Escherichia coli K-12: isolation and mapping.
    J Bacteriol. 1975 Oct;124(1):26-38 PMID: 1100602
  2. Genotypic exclusion: a novel relationship between the ribitol-arabitol and galactitol genes of E. coli.
    Mol Gen Genet. 1983;189(2):337-9 PMID: 6343795
  3. The gene for ribosomal protein L25 (rplY) maps at 47.3 min near nalA in Escherichia coli K-12.
    Mol Gen Genet. 1979 Nov;176(3):313-8 PMID: 392236
  4. Purification of the colicin I receptor.
    J Biol Chem. 1983 Jan 25;258(2):1215-20 PMID: 6337134
  5. cir, a gene conferring resistance to colicin I maps between mgl and fpk on the Escherichia coli chromosome.
    Mol Gen Genet. 1983;191(3):401-6 PMID: 6314091
  6. Lambda placMu: a transposable derivative of bacteriophage lambda for creating lacZ protein fusions in a single step.
    J Bacteriol. 1984 Jun;158(3):1084-93 PMID: 6327627
  7. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition.
    J Mol Biol. 1975 Oct 5;97(4):561-75 PMID: 1102715
  8. Mapping of mglB, the structural gene of the galactose-binding protein of Escherichia coli.
    Mol Gen Genet. 1981;184(3):508-18 PMID: 6278261
  9. Extent of host deletions associated with bacteriophage P2-mediated eduction.
    J Bacteriol. 1971 Nov;108(2):695-704 PMID: 4942760
  10. A second transport system for sn-glycerol-3-phosphate in Escherichia coli.
    J Bacteriol. 1978 Dec;136(3):1070-83 PMID: 363686
  11. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  12. Mapping of the gene for cytidine deaminase (cdd) in Escherichia coli K-12.
    J Bacteriol. 1983 Apr;154(1):72-5 PMID: 6339482
  13. Hfr formation directed by tn10.
    Genetics. 1979 Apr;91(4):639-55 PMID: 17248903
  14. Isolation and behavior of Escherichia coli deletion mutants lacking chemotaxis functions.
    J Bacteriol. 1982 Jul;151(1):106-13 PMID: 7045071
  15. Genetical analysis of fructose utilization by Escherichia coli.
    Proc R Soc Lond B Biol Sci. 1974 Sep 17;187(1087):121-31 PMID: 4154035
  16. Mutants of Escherichia coli unable to metabolize cytidine: isolation and characterization.
    Mol Gen Genet. 1973 Nov 2;126(2):177-86 PMID: 4360102
  17. Only one gene is required for the glpT-dependent transport of sn-glycerol-3-phosphate in Escherichia coli.
    Mol Gen Genet. 1982;186(4):540-7 PMID: 6752662
  18. The role of phosphotransferase-mediated syntheses of fructose 1-phosphate and fructose 6-phosphate in the growth of Escherichia coli on fructose.
    Proc R Soc Lond B Biol Sci. 1974 Sep 17;187(1087):105-19 PMID: 4153999
  19. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
    J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
  20. Mapping of nrdA and nrdB in Escherichia coli K-12.
    J Bacteriol. 1976 Dec;128(3):810-4 PMID: 791934
  21. Construction of an Escherichia coli strain unable to synthesize putrescine, spermidine, or cadaverine: characterization of two genes controlling lysine decarboxylase.
    J Bacteriol. 1980 Dec;144(3):952-6 PMID: 7002915
  22. Altered deoxyribonucleotide pools in P2 eductants of Escherichia coli K-12 due to deletion of the dcd gene.
    J Bacteriol. 1976 May;126(2):999-1001 PMID: 177407
  23. High-affinity arabinose transport mutants of Escherichia coli: isolation and gene location.
    J Bacteriol. 1981 Sep;147(3):920-4 PMID: 7024251
  24. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
    J Mol Biol. 1976 Jul 5;104(3):541-55 PMID: 781293
  25. Genetic mapping of a mutation that causes ribonucleases III deficiency in Escherichia coli.
    J Bacteriol. 1975 Oct;124(1):307-16 PMID: 1100605
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327939
Published
1984-00-00
Pages
175-81
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327939
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2