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PMID: 177407 Published · ppublish English Journal Article

Altered deoxyribonucleotide pools in P2 eductants of Escherichia coli K-12 due to deletion of the dcd gene.

Journal of bacteriology ·Vol. 126 ·No. 2 ·1976-05-00 ·Pages 999-1001

Neuhard J, Thomassen E

Abstract

Deletion of the Escherichia coli K-12 chromosome associated with P2 mediated education extend through the structural gene for uridine kinase, udk, and the dcd gene encoding 2'-deoxycytidine 5'-triphosphate deaminase. The lack of uridine kinase makes a positive selection possible for these strains. Due to the dcd mutation, P2 eductants show large alterations in their deoxyribonucleoside triphosphate pools.

MeSH Terms
Deoxyribonucleotides/metabolism Escherichia coli/enzymology,metabolism Genes Histidine/metabolism Mutation Nucleotide Deaminases/biosynthesis Phosphotransferases/biosynthesis Transduction, Genetic Uridine Kinase/biosynthesis
Chemicals
Deoxyribonucleotides Histidine Phosphotransferases Uridine Kinase Nucleotide Deaminases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Neuhard J
Thomassen E
References (12)
12 references, click to expand
  1. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  2. Deoxycytidine triphosphate deaminase of Salmonella typhimurium. Purification and characterization.
    J Biol Chem. 1975 Jan 25;250(2):609-16 PMID: 234434
  3. Metabolism of pyrimidines and pyrimidine nucleosides by Salmonella typhimurium.
    J Bacteriol. 1972 Apr;110(1):219-28 PMID: 4259664
  4. Mutants of Escherichia coli unable to metabolize cytidine: isolation and characterization.
    Mol Gen Genet. 1973 Nov 2;126(2):177-86 PMID: 4360102
  5. Location on the chromosome of Salmonella typhimurium of genes governing pyrimidine metabolism. II. Uridine kinase, cytosine deaminase and thymidine kinase.
    Mol Gen Genet. 1972;115(3):208-15 PMID: 4555675
  6. Effect of thymine concentration on the replication velocity of DNA in a thymineless mutant of Escherichia coli.
    Nature. 1970 Apr 11;226(5241):126-31 PMID: 4908371
  7. The role of nucleoside phosphorylases in the degradation of deoxyribonucleosides by thymine-requiring mutants of E. coli.
    Mol Gen Genet. 1971;110(4):289-98 PMID: 4930081
  8. Turnover of the deoxyribonucleoside triphosphates in Escherichia coli 15 T during thymine starvation.
    Eur J Biochem. 1971 May 11;20(1):36-43 PMID: 4931186
  9. Extent of host deletions associated with bacteriophage P2-mediated eduction.
    J Bacteriol. 1971 Nov;108(2):695-704 PMID: 4942760
  10. Synthesis and breakdown of messenger RNA without protein synthesis.
    J Mol Biol. 1966 Feb;15(2):428-34 PMID: 5330747
  11. Deoxycytidine triphosphate deaminase: characterization of an Escherichia coli mutant deficient in the enzyme.
    J Bacteriol. 1971 Feb;105(2):666-72 PMID: 5541540
  12. Ion-exchange thin-layer chromatography. XV. Preparation, properties and applications of paper-like PEI-cellulose sheets.
    J Chromatogr. 1966 Apr;22(1):110-7 PMID: 5922282
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.126.2.999-1001.1976
Published
1976-05-00
Pages
999-1001
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233240
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.126.2.999-1001.1976 PMC Full Text View journal details
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