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PMID: 6392014 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Specific deletion occurring in the directed evolution of 6-phosphogluconate dehydrogenase in Escherichia coli.

Genetics ·Vol. 108 ·No. 4 ·1984-12-00 ·Pages 765-72

Miller RD, Dykhuizen DE, Green L, Hartl DL

Abstract

A novel genetic change leading to increased activity of 6-phosphogluconate dehydrogenase (6PGD) in E. coli has been observed. The mutation is a deletion of approximately 0.4 kilobase pairs occurring between the structural gene of 6PGD (gnd) and one copy of an insertion element (IS5) found normally in E. coli K12 a few hundred base pairs upstream (counterclockwise) from gnd at 44 minutes on the conventional genetic map. The deletion is associated with a threefold higher activity of 6PGD and a 57% increase in the maximum growth rate when cells are grown in gluconate.

MeSH Terms
Bacterial Proteins/genetics Chromosome Deletion Chromosomes, Bacterial/ultrastructure Escherichia coli/enzymology,genetics Mutation Phosphogluconate Dehydrogenase/genetics Selection, Genetic
Chemicals
Bacterial Proteins Phosphogluconate Dehydrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Miller R D
Dykhuizen D E
Green L
Hartl D L
References (8)
8 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/108.4.765
Published
1984-12-00
Pages
765-72
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1224263
Subset
IM
Grants
NIGMS NIH HHS · GM30201 · United States
External Links
PubMed source DOI: 10.1093/genetics/108.4.765 PMC Full Text View journal details
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