Mutation to rifampicin resistance at the beginning of the RNA polymerase beta subunit gene in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 196 ·No. 1 ·1984-00-00 ·Pages 173-4

Lisitsyn NA, Sverdlov ED, Moiseyeva EP, Danilevskaya ON, Nikiforov VG

Abstract

The unusual recombinant plasmid pRC19 carrying the N-terminal fragment of the Escherichia coli RNA polymerase rpoB gene was found to specify high level rifampicin resistance of E. coli cells. Sequence analysis of this plasmid revealed one substitution only: transversion G----T, leading to amino acid substitution Val146----Phe. This mutational change marks the second domain of the beta subunit involved in rifampicin binding.

MeSH Terms

Base Sequence DNA-Directed RNA Polymerases/genetics Drug Resistance, Microbial Escherichia coli/genetics Genes Genes, Bacterial Macromolecular Substances Mutation Rifampin/pharmacology

Chemicals

Macromolecular Substances DNA-Directed RNA Polymerases Rifampin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Lisitsyn N A

Sverdlov E D

Moiseyeva E P

Danilevskaya O N

Nikiforov V G

References (7)

7 references, click to expand

[Genetic study of mutations changing the dominance of alleles of sensitivity of Escherichia coli K-12 RNA polymerase to rifampicin and streptolydigin].
Genetika. 1973 Dec;9(12):84-94 PMID: 4619996
Genetic studies on the beta subunit of Escherichia coli RNA polymerase. III. Analysis of low-level rifampicin-resistant mutants.
Mol Gen Genet. 1983;190(3):523-6 PMID: 6348484
RNA polymerase rifampicin resistance mutations in Escherichia coli: sequence changes and dominance.
Mol Gen Genet. 1983;190(2):344-8 PMID: 6348476
The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.
Eur J Biochem. 1981 Jun 1;116(3):621-9 PMID: 6266829
Primary structure of an EcoRI fragment of lambda imm434 DNA containing regions cI-cro of phage 434 and cII-o of phage lambda.
Gene. 1979 Jul;6(3):235-49 PMID: 478301
Primary structure of Escherichia coli RNA polymerase nucleotide substitution in the beta subunit gene of the rifampicin resistant rpoB255 mutant.
Mol Gen Genet. 1981;184(3):536-8 PMID: 6278262
[Nucleotide substitutions in the rpoB gene leading to rifampicin resistance of E. coli RNA polymerase].
Bioorg Khim. 1984 Jan;10(1):127-8 PMID: 6385989

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00334112

Published

1984-00-00

Pages

173-4

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00334112

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Mutation to rifampicin resistance at the beginning of the RNA polymerase beta subunit gene in Escherichia coli.

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