Primary structure of Escherichia coli RNA polymerase nucleotide substitution in the beta subunit gene of the rifampicin resistant rpoB255 mutant.

Molecular & general genetics : MGG ·Vol. 184 ·No. 3 ·1981-00-00 ·Pages 536-8

Ovchinnikov YuA, Monastyrskaya GS, Gubanov VV, Lipkin VM, Sverdlov ED, Kiver IF, Bass IA, Mindlin SZ, Danilevskaya ON, Khesin RB

Abstract

The transducing phage lambda dsupM814 and the plasmid pIB1830 containing the wild-type rpoB gene have been constructed and the primary structure of the gene's central fragment has been established. In contrast with the wild-type, the gene of the rpoB255 mutant, whose primary structure has been published, was found to contain an A.T. leads to T.A. transversion entailing the substitution of a valine residue for the aspartic acid residue (516) of the wild-type beta subunit.

MeSH Terms

Base Sequence DNA Restriction Enzymes DNA, Bacterial/genetics DNA-Directed RNA Polymerases/genetics Drug Resistance, Microbial Escherichia coli/enzymology,genetics Genes/drug effects Mutation Plasmids Rifampin/pharmacology Transduction, Genetic

Chemicals

DNA, Bacterial DNA-Directed RNA Polymerases DNA Restriction Enzymes Rifampin

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Ovchinnikov YuA

Monastyrskaya G S

Gubanov V V

Lipkin V M

Sverdlov E D

Kiver I F

Bass I A

Mindlin S Z

Danilevskaya O N

Khesin R B

References (10)

10 references, click to expand

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RNA polymerase mutants of Escherichia coli. Streptolydigin resistance and its relation to rifampicin resistance.
Mol Gen Genet. 1973 Mar 1;121(2):181-96 PMID: 4571524
The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.
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Synthesis of ribosomal RNA in E. coli: analysis using deletion mutants of a lambda transducing phage carrying ribosomal RNA genes.
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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00352535

Published

1981-00-00

Pages

536-8

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00352535

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Primary structure of Escherichia coli RNA polymerase nucleotide substitution in the beta subunit gene of the rifampicin resistant rpoB255 mutant.

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