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PMID: 6374373 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

P. mirabilis RecA protein catalyses cleavage of E. coli LexA protein and the lambda repressor in vitro.

Molecular & general genetics : MGG ·Vol. 194 ·No. 1-2 ·1984-00-00 ·Pages 111-3

West SC, Little JW

Abstract

The cloned recA+ gene of proteus mirabilis substitutes for a defective RecA protein in Escherichia coli recA- mutants, and restores recombination, repair and phage induction functions to near normal levels. In a previous report, we described the purification and characterisation of the recombination activities of the P. mirabilis RecA protein (West et al. 1983b ). In this paper, we show that the purified protein catalyses the cleavage of both the Escherichia coli LexA protein and the bacteriophage lambda repressor in vitro. These results provide a direct biochemical basis for the interspecies complementation observed in vivo and suggest that P. mirabilis has an SOS regulatory network similar to that of E. coli.

MeSH Terms
Bacterial Proteins/metabolism DNA Repair DNA, Bacterial/metabolism DNA-Binding Proteins Escherichia coli/genetics,metabolism Protein Processing, Post-Translational Proteus mirabilis/genetics Rec A Recombinases/genetics,physiology Repressor Proteins/metabolism Serine Endopeptidases Species Specificity Transcription Factors/metabolism Viral Proteins Viral Regulatory and Accessory Proteins
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins LexA protein, Bacteria Repressor Proteins Transcription Factors Viral Proteins Viral Regulatory and Accessory Proteins phage repressor proteins Rec A Recombinases Serine Endopeptidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
West S C
Little J W
References (15)
15 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00383505
Published
1984-00-00
Pages
111-3
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIADDK NIH HHS · AM 09397 · United States
NCI NIH HHS · CA 26763 · United States
NIGMS NIH HHS · GM 11014 · United States
External Links
PubMed source DOI: 10.1007/BF00383505
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