Identification of a cDNA clone encoding a mature blood stage antigen of Plasmodium falciparum by immunization of mice with bacterial lysates.
A cDNA library was constructed in pBR322 using mRNA from blood stages of a Papua New Guinean isolate of Plasmodium falciparum. Expression of parasite antigens was not directly detectable by conventional immunological assays. To circumvent this, mice were immunized with lysates of cDNA clones, and the antisera raised were assayed for anti-parasite reactivity. One cDNA clone was identified which reliably elicited antibodies to P. falciparum. The mouse antisera were used to characterize the native P. falciparum protein as a 120-kd protein, which is antigenic during natural infection. The protein occurs in late trophozoite and schizont stages and is found in isolates of the parasite from widely separated geographical areas. The genomic context of the antigen gene is conserved in the different isolates.
18 references, click to expand
-
Maturation of the head of bacteriophage T4. I. DNA packaging events.J Mol Biol. 1973 Nov 15;80(4):575-99 PMID: 4204102
-
A simple method for the preparation of large quantities of pure plasmid DNA.Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
-
High resolution two-dimensional electrophoresis of proteins.J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
-
Detection of specific sequences among DNA fragments separated by gel electrophoresis.J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
-
Human malaria parasites in continuous culture.Science. 1976 Aug 20;193(4254):673-5 PMID: 781840
-
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
-
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.Gene. 1977;2(2):95-113 PMID: 344137
-
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.Gene. 1979 May;6(1):23-8 PMID: 383576
-
Synchronization of Plasmodium falciparum erythrocytic stages in culture.J Parasitol. 1979 Jun;65(3):418-20 PMID: 383936
-
Molecular cloning of seven mouse immunoglobulin kappa chain messenger ribonucleic acids.Biochemistry. 1980 Jun 10;19(12):2702-10 PMID: 6249337
-
Inhibition of P. falciparum growth in human erythrocytes by monoclonal antibodies.Nature. 1981 Jan 22;289(5795):301-3 PMID: 6161311
-
Production of immunologically active surface antigens of hepatitis B virus by Escherichia coli.Proc Natl Acad Sci U S A. 1981 Jul;78(7):4510-4 PMID: 6170067
-
Direct immunoassay for detecting Escherichia coli colonies that contain polypeptides encoded by cloned DNA segments.Proc Natl Acad Sci U S A. 1981 Jul;78(7):4520-4 PMID: 6794032
-
Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody.J Exp Med. 1982 Nov 1;156(5):1528-38 PMID: 6752328
-
Cloning and expression in E. coli of the malarial sporozoite surface antigen gene from Plasmodium knowlesi.Nature. 1983 Apr 7;302(5908):536-8 PMID: 6339949
-
Differential effect of immunoglobulin on the in vitro growth of several isolates of Plasmodium falciparum.Infect Immun. 1983 Mar;39(3):1228-35 PMID: 6188695
-
Expression of Plasmodium falciparum blood-stage antigens in Escherichia coli: detection with antibodies from immune humans.Proc Natl Acad Sci U S A. 1983 Jun;80(12):3787-91 PMID: 6304737
-
Antigens of the erythrocytes stages of the human malaria parasite Plasmodium falciparum detected by monoclonal antibodies.Mol Biochem Parasitol. 1983 Mar;7(3):247-65 PMID: 6350871
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
