Home LiteratureArticle Details
PMID: 6361500 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Promoter selectivity of Escherichia coli RNA polymerase. II: Altered promoter selection by mutant holoenzymes.

Molecular & general genetics : MGG ·Vol. 193 ·No. 1 ·1984-00-00 ·Pages 8-16

Nomura T, Ishihama A, Kajitani M, Takahashi T, Nakada N, Yoshinaga K

Abstract

Using the in vitro mixed transcription system (Kajitani and Ishihama (1983a, 1983b), we examined selective transcription of truncated DNA templates carrying lac(UV5), rrnE or rpsA promoters by RNA polymerase holoenzymes from pairs of wild-type parents and mutants with a mutation in one or more RNA polymerase subunit genes. The promoter selectivity of RNA polymerases from two sigma-subunit mutants carrying either rpoD2 or rpoD285 differed markedly from that of the respective wild-type enzymes. Both the parental RNA polymerases, however, exhibited abnormal promoter selectivity compared with holoenzymes from various wild-type E. coli strains. On the other hand, all the RNA polymerases from rpoB and/or rpoC mutants and the respective wild-type parents were similar, if not identical, in promoter selection at low temperature. At high temperature, however, RNA polymerases from mutants carrying rpoB2B7 and rpoC4, affecting the beta and beta' subunits, respectively, showed decreased transcription from the high-affinity slow-transcribable promoter rrnEp2 whereas the rpoC92 and rpoB906 X rpoC907 mutant enzymes both lost transcription activity from the strong promoter lacP(UV5). Taking all these observations together we conclude that not only the sigma subunit but also the beta and beta' subunits are involved in the recognition of promoters.

MeSH Terms
DNA-Directed RNA Polymerases/genetics Escherichia coli/enzymology,genetics Genotype Kinetics Mutation Operon Templates, Genetic Transcription, Genetic
Chemicals
DNA-Directed RNA Polymerases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nomura T
Ishihama A
Kajitani M
Takahashi T
Nakada N
Yoshinaga K
References (38)
38 references, click to expand
  1. Physiological studies on a temperature-sensitive Escherichia coli mutant with an altered RNA polymerase beta'-subunit.
    Biochim Biophys Acta. 1977 Nov 16;479(2):172-9 PMID: 336096
  2. The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit.
    Nucleic Acids Res. 1982 Jul 10;10(13):4035-44 PMID: 6287430
  3. A mutation affecting the sigma subunit of RNA polymerase changes transcriptional specificity.
    Nature. 1978 Jun 1;273(5661):354-8 PMID: 351406
  4. Temperature-sensitive Escherichia coli mutant producing a temperature-sensitive sigma subunit of DNA-dependent RNA polymerase.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):6177-81 PMID: 366614
  5. Determination of the promoter strength in the mixed transcription system. II. Promoters of ribosomal RNA, ribosomal protein S1 and recA protein operons from Escherichia coli.
    Nucleic Acids Res. 1983 Jun 25;11(12):3873-88 PMID: 6346267
  6. Suppression of the DnaA phenotype by mutations in the rpoB cistron of ribonucleic acid polymerase in Salmonella typhimurium and Escherichia coli.
    J Bacteriol. 1977 May;130(2):577-82 PMID: 400782
  7. Biosynthesis of RNA polymerase in Escherichia coli. IX. Growth-dependent variations in the synthesis rate, content and distribution of RNA polymerase.
    Mol Gen Genet. 1979 Jul 13;174(2):107-16 PMID: 386039
  8. Glutamine synthetase in preparations of RNA polymerase of Escherichia coli: a novel purification procedure.
    J Biochem. 1982 May;91(5):1719-24 PMID: 6124539
  9. Genetics of bacterial RNA polymerases.
    Annu Rev Genet. 1979;13:59-97 PMID: 94253
  10. Biosynthesis of RNA polymerase in Escherichia coli. XII. Noncoordinate synthesis of core enzyme subunits after suppression of cell growth.
    Mol Gen Genet. 1982;185(3):373-8 PMID: 7048023
  11. Correlation between the serine sensitivity and the derepressibility of the ilv genes in Escherichia coli relA- mutants.
    Mol Gen Genet. 1978 Sep 20;165(1):21-30 PMID: 362163
  12. Relationship between the N function of bacteriophage lambda and host RNA polymerase.
    J Mol Biol. 1972 Mar 28;65(2):259-72 PMID: 5040359
  13. Host mutant (tabD)-induced inhibition of bacteriophage T4 late transcription. I. Isolation and phenotypic characterization of the mutants.
    J Mol Biol. 1975 Aug 25;96(4):579-600 PMID: 1195367
  14. A simple procedure for resolution of Escherichia coli RNA polymerase holoenzyme from core polymerase.
    Arch Biochem Biophys. 1977 Aug;182(2):404-8 PMID: 332084
  15. Primary structure of alpha-subunit of DNA-dependent RNA polymerase from Escherichia coli.
    FEBS Lett. 1977 Apr 1;76(1):108-11 PMID: 323055
  16. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
    Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
  17. Determination of the promoter strength in the mixed transcription system: promoters of lactose, tryptophan and ribosomal protein L10 operons from Escherichia coli.
    Nucleic Acids Res. 1983 Feb 11;11(3):671-86 PMID: 6300761
  18. The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.
    Eur J Biochem. 1981 Jun 1;116(3):621-9 PMID: 6266829
  19. A link between streptomycin and rifampicin mutation.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2084-7 PMID: 1094452
  20. Ribonucleic acid polymerase mutant of Escherichia coli defective in flagella formation.
    J Bacteriol. 1977 Oct;132(1):254-61 PMID: 199575
  21. Subunit of assembly of Escherichia coli RNA polymerase.
    Adv Biophys. 1981;14:1-35 PMID: 7015808
  22. Non-coordinate synthesis of RNA polymerase beta beta' subunits in a temperature-sensitive beta'-subunit mutant of Escherichia coli.
    Mol Gen Genet. 1982;188(2):173-8 PMID: 6759869
  23. Alteration of regulation of arginine biosynthesis in Escherichia coli W by mutation to rifampin resistance.
    Biochim Biophys Acta. 1975 Feb 24;383(1):106-16 PMID: 1091297
  24. A temperature-sensitive mutant of Escherichia coli with an altered RNA polymerase beta' subunit.
    Biochem Biophys Res Commun. 1977 Jun 6;76(3):739-45 PMID: 332156
  25. ALT: a new factor involved in the synthesis of RNA by Escherichia coli.
    Mol Gen Genet. 1972;118(3):223-34 PMID: 4343249
  26. A procedure for the rapid purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
    Biochem J. 1973 May;133(1):201-3 PMID: 4578761
  27. Conditional change of DNA replication control in an RNA polymerase mutant of Escherichia coli.
    J Bacteriol. 1983 Apr;154(1):443-51 PMID: 6339480
  28. Interaction between mutations of ribosomes and RNA polymerase: a pair of strA and rif mutants individually temperature-insensitive but temperature-sensitive in combination.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1157-61 PMID: 322146
  29. Temperature-sensitive RNA polymerase mutants with altered subunit synthesis and degradation.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2375-9 PMID: 1094466
  30. Construction of plasmids carrying the cI gene of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4174-8 PMID: 1069307
  31. Temperature-sensitive mutations in the alpha subunit gene of Escherichia coli RNA polymerase.
    J Mol Biol. 1980 Feb 25;137(2):137-50 PMID: 6154799
  32. Interaction of RNA polymerase mutations in haploid and merodiploid cells of Escherichia coli K-12.
    Mol Gen Genet. 1971;110(2):118-33 PMID: 4927611
  33. Biosynthesis of RNA polymerase in Escherichia coli. IV. Accumulation of intermediates in mutants defective in the subunit assembly.
    J Mol Biol. 1976 Apr 5;102(2):297-310 PMID: 775104
  34. The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12.
    Nucleic Acids Res. 1981 Jun 25;9(12):2889-903 PMID: 6269063
  35. Biosynthesis of RNA polymerase in Escherichia coli. V. Defects of the subunit assembly in a temperature-sensitive beta subunit mutant.
    J Mol Biol. 1977 May 5;112(1):65-74 PMID: 328895
  36. Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):2977-81 PMID: 4943550
  37. An RNA polymerase mutant of Escherichia coli defective in the T4 viral transcription program.
    Virology. 1972 Nov;50(2):396-403 PMID: 4564523
  38. Altered synthesis and stability of RNA polymerase holoenzyme subunits in mutants of Escherichia coli with mutations in the beta or beta' subunit genes.
    Mol Gen Genet. 1976 Aug 19;147(2):139-43 PMID: 787754
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327407
Published
1984-00-00
Pages
8-16
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327407
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2