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PMID: 6350846 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Maturation of a 100 kDa protein associated with preribosomes in Chinese hamster ovary cells.

Molecular biology reports ·Vol. 9 ·No. 1-2 ·1983-05-00 ·Pages 39-47

Bourbon HM, Bugler B, Caizergues-Ferrer M, Amalric F, Zalta JP

Abstract

A 100 kDa nucleolar protein which is transitorily associated with preribosomes in the nucleoli of Chinese hamster ovary cells has been found to be specifically cleaved by a thiol protease. During an 'in vitro' incubation of nucleoli, the 100 kDa protein is processed into eight different proteins which are detected by immunoreaction with a serum raised against the 100 kDa protein. Qualitative and quantitative variations in the maturation products of the 100 kDa protein are obtained by 'in vitro' incubation of the 60S and 80S preribosomes. The 100 kDa protein has been purified to homogeneity with the protease activity still associated. The properties of the enzyme are described and its role in the maturation of preribosomes is discussed.

MeSH Terms
Animals Cell Line Cell Nucleolus/enzymology,metabolism Cricetinae Cysteine Endopeptidases Endopeptidases/metabolism Molecular Weight Morphogenesis Peptide Hydrolases/metabolism Protein Precursors/metabolism Ribosomal Proteins/metabolism Ribosomes/metabolism,ultrastructure
Chemicals
Protein Precursors Ribosomal Proteins Endopeptidases Peptide Hydrolases Cysteine Endopeptidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bourbon H M
Bugler B
Caizergues-Ferrer M
Amalric F
Zalta J P
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28 references, click to expand
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Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00777472
Published
1983-05-00
Pages
39-47
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00777472 View journal details
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