Isolation of nucleoli. A method that combines high yield, structural integrity, and biochemical preservation.

The Journal of cell biology ·Vol. 51 ·No. 21 ·1971-11-00 ·Pages 563-8

Zalta J, Zalta JP, Simard R

Abstract

暂无摘要

MeSH Terms

Animals Ascitic Fluid Cell Fractionation Cell Nucleolus/analysis Cells, Cultured Centrifugation, Density Gradient Chromatin/analysis DNA, Neoplasm/analysis Electrophoresis Female Histocytochemistry Methods Microscopy, Electron Neoplasm Proteins/analysis Pepsin A Peritoneal Neoplasms RNA, Neoplasm/analysis,isolation & purification Rats Ribonucleases Vibration

Chemicals

Chromatin DNA, Neoplasm Neoplasm Proteins RNA, Neoplasm Ribonucleases Pepsin A

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Zalta J

Zalta J P

Simard R

References (17)

17 references, click to expand

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Recognition of ribosomal RNA sites in DNA. II. The HeLa cell system.
Proc Natl Acad Sci U S A. 1965 Jul;54(1):185-92 PMID: 5216350
Small molecular weight monodisperse nuclear RNA.
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J Cell Biol. 1963 Aug;18:293-312 PMID: 14079490
The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.
Biochem J. 1969 Jun;113(1):131-8 PMID: 4896715
Processing of 45 s nucleolar RNA.
J Mol Biol. 1970 Jan 28;47(2):169-78 PMID: 4314037
Electrophoretic comparison of nuclear and nucleolar proteins. I. Beef pancreas.
Biochim Biophys Acta. 1961 Jan 15;46:373-80 PMID: 13737073
[Effect of supra-optimal temperature on nucleolar ribonucleoproteins and RNA. II. Biochemical study].
Exp Cell Res. 1969 Jun;55(3):370-7 PMID: 4306776
THE RELATIONSHIP OF THE NUCLEOLUS TO THE SYNTHESIS OF RIBOSOMAL RNA IN HELA CELLS.
Proc Natl Acad Sci U S A. 1964 Jun;51:1197-204 PMID: 14215644
STUDIES ON ISOLATED NUCLEI. I. ISOLATION AND CHEMICAL CHARACTERIZATION OF A NUCLEAR FRACTION FROM GUINEA PIG LIVER.
J Cell Biol. 1963 Aug;18:267-91 PMID: 14079489
Nuclei from rat liver: isolation method that combines purity with high yield.
Science. 1966 Dec 30;154(3757):1662-5 PMID: 5924199
Common features of the base composition of rapidly labeled RNA of nucleoli in a number of experimental tumors.
Cancer Res. 1967 Jun;27(6):1084-91 PMID: 4290859
Isolation and fractionation of nucleic acids.
Prog Nucleic Acid Res Mol Biol. 1964;3:1-31 PMID: 5318915
Isolation and properties of liver cell nucleoli.
J Biophys Biochem Cytol. 1956 Mar 25;2(2):127-45 PMID: 13319377
Isolation of pure and unaltered liver nuclei morphology and biochemical composition.
Exp Cell Res. 1956 Aug;11(2):317-21 PMID: 13375653
The Isolation and Chemical Properties of the Nucleoli of Starfish Oocytes.
Proc Natl Acad Sci U S A. 1952 Feb;38(2):139-45 PMID: 16589069
ISOLATION OF NUCLEOLI OF THE WALKER CARCINOSARCOMA AND LIVER OF THE RAT FOLLOWING NUCLEAR DISRUPTION IN A FRENCH PRESSURE CELL.
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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.51.2.563

Published

1971-11-00

Pages

563-8

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2108127

Subset

External Links

PubMed source DOI: 10.1083/jcb.51.2.563 PMC Full Text View journal details

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Isolation of nucleoli. A method that combines high yield, structural integrity, and biochemical preservation.

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