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PMID: 6344792 Published · ppublish English Comparative Study Journal Article

Foodborne enterotoxigenic Escherichia coli: detection and enumeration by DNA colony hybridization.

Applied and environmental microbiology ·Vol. 45 ·No. 4 ·1983-04-00 ·Pages 1324-30

Hill WE, Madden JM, McCardell BA, Shah DB, Jagow JA, Payne WL, Boutin BK

Abstract

Four methods were compared for detecting heat-labile toxin production by Escherichia coli: DNA colony hybridization, two enzyme-linked immunosorbent assays, and the mouse Y-1 adrenal cell reaction. Although results of the methods were in general agreement, there were some differences in specificity and sensitivity. DNA colony hybridization was used to detect and enumerate enterotoxigenic E. coli isolates in artificially contaminated food without enrichment. Sensitivity level was 100 cells per g.

MeSH Terms
Bacterial Toxins Bacteriological Techniques Biological Assay Enterotoxins/analysis,biosynthesis,genetics Enzyme-Linked Immunosorbent Assay Escherichia coli/genetics,growth & development,metabolism Escherichia coli Proteins Food Microbiology Nucleic Acid Hybridization
Chemicals
Bacterial Toxins Enterotoxins Escherichia coli Proteins heat-labile enterotoxin, E coli
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hill W E
Madden J M
McCardell B A
Shah D B
Jagow J A
Payne W L
Boutin B K
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48 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.45.4.1324-1330.1983
Published
1983-04-00
Pages
1324-30
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC242458
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.45.4.1324-1330.1983 PMC Full Text View journal details
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