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PMID: 6344022 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of human interferon genes using the recA promoter of Escherichia coli.

Nucleic acids research ·Vol. 11 ·No. 9 ·1983-05-11 ·Pages 2927-41

Feinstein SI, Chernajovsky Y, Chen L, Maroteaux L, Mory Y

Abstract

Interferon beta 1 and three alpha-interferon genes were cloned on Eco RI fragments isolated from a human genomic library into the Eco RI site of a plasmid containing the recA promoter of E. coli. Expression of interferon activity from cells carrying these plasmids was nalidixic acid inducible. The alpha-interferon genes were expressed only when in the same transcriptional orientation as the recA promoter while the beta 1 interferon gene was expressed in either orientation. Interferon activity was also inducibly expressed from the recA promoter in cells containing a plasmid carrying a fusion of the recA gene with the beta 1 interferon gene. This interferon activity was thirty-fold less sensitive to neutralization by polyclonal antibodies than authentic interferon, implying that the change near the amino terminus affects either antibody recognition or specific activity or both.

MeSH Terms
Bacterial Proteins/genetics Cloning, Molecular Escherichia coli/genetics Gene Expression Regulation Humans Interferon Type I/genetics Operon Plasmids Rec A Recombinases
Chemicals
Bacterial Proteins Interferon Type I Rec A Recombinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Feinstein S I
Chernajovsky Y
Chen L
Maroteaux L
Mory Y
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46 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.9.2927
Published
1983-05-11
Pages
2927-41
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325934
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.9.2927 PMC Full Text View journal details
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