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PMID: 6340114 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

An effect of nerve growth factor on parasympathetic neurite outgrowth.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 7 ·1983-04-00 ·Pages 2091-4

Collins F, Dawson A

Abstract

Addition of nerve growth factor (NGF) to dissociated parasympathetic ciliary ganglion neurons resulted, within 60 min of its addition, in a 2-fold increase in average neurite length and an accompanying enlargement and spreading of neuronal growth cones. These effects occurred over a concentration range of NGF of 0.1-10 ng/ml and were blocked by affinity-purified antibody to NGF. Epidermal growth factor, fibroblast growth factor, and angiotensin did not have these effects, although insulin at high concentrations was able to induce a response similar to that of NGF. Dissociated sympathetic chain neurons also responded to NGF with increased neurite lengths, and, in addition, NGF considerably extended the survival time of these neurons in culture. However, the effect of NGF on ciliary ganglion neurons was limited to neurite outgrowth, and NGF did not promote the survival of these parasympathetic neurons.

MeSH Terms
Animals Cell Differentiation/drug effects Cell Survival/drug effects Cells, Cultured Culture Media Insulin/pharmacology Mice Nerve Growth Factors/pharmacology Parasympathetic Nervous System/cytology,growth & development
Chemicals
Culture Media Insulin Nerve Growth Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Collins F
Dawson A
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.7.2091
Published
1983-04-00
Pages
2091-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393759
Subset
IM
Grants
NINDS NIH HHS · NS15130 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.7.2091 PMC Full Text View journal details
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