A type II restriction endonuclease with an eight nucleotide specificity from Streptomyces fimbriatus.

Nucleic acids research ·Vol. 12 ·No. 11 ·1984-06-11 ·Pages 4507-16

Qiang BQ, Schildkraut I

Abstract

A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus . This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN / NGGCC , symmetrically to produce a three base, 3' extension.

MeSH Terms

Base Sequence Cloning, Molecular DNA Restriction Enzymes/isolation & purification,metabolism DNA, Viral Deoxyribonucleases, Type II Site-Specific Genetic Vectors Kinetics Streptomyces/enzymology Substrate Specificity T-Phages

Chemicals

DNA, Viral DNA Restriction Enzymes endodeoxyribonuclease SfiI Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Qiang B Q

Schildkraut I

References (17)

17 references, click to expand

Cleavage specificity of the restriction endonuclease isolated from Haemophilus gallinarum (Hga I).
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3213-6 PMID: 269384
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
The genome of simian virus 40.
Science. 1978 May 5;200(4341):494-502 PMID: 205947
The nucleotide sequence of bacteriophage phiX174.
J Mol Biol. 1978 Oct 25;125(2):225-46 PMID: 731693
A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13.
J Mol Biol. 1979 Mar 25;129(1):169-72 PMID: 448736
Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
The specific non-symmetrical sequence recognized by restriction endonuclease MboII.
J Mol Biol. 1980 Jun 15;140(1):143-8 PMID: 6251231
Identification of palindromic sequences recognized by restriction endonucleases, as based on the tabularized sequencing data for seven viral and plasmid DNAs.
Gene. 1980 Sep;10(4):357-70 PMID: 6253357
Correlation between genetic map and map of cleavage sites for sequence-specific endonucleases SalI, KpnI, BglI, and BamHI in bacteriophage T4 cytosine-containing DNA.
J Virol. 1980 Oct;36(1):1-17 PMID: 6255198
A system for shotgun DNA sequencing.
Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
Two new restriction endonucleases from Proteus vulgaris.
Nucleic Acids Res. 1981 Sep 25;9(18):4525-36 PMID: 6272209
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
Gene. 1982 Oct;19(3):269-76 PMID: 6295880
Restriction endonuclease BglI as a tool for in vitro reconstruction and recombination of plasmid and bacteriophage genomes.
Mol Gen Genet. 1983;189(2):269-74 PMID: 6304467
New techniques for purifying large DNAs and studying their properties and packaging.
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:189-95 PMID: 6222863
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.
J Mol Biol. 1983 Jun 5;166(4):477-535 PMID: 6864790
Restriction and modification enzymes and their recognition sequences.
Nucleic Acids Res. 1983 Jan 11;11(1):r135-67 PMID: 6306557
Structure of the human immunoglobulin mu locus: characterization of embryonic and rearranged J and D genes.
Cell. 1981 Dec;27(3 Pt 2):583-91 PMID: 6101209

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.11.4507

Published

1984-06-11

Pages

4507-16

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318854

Subset

External Links

PubMed source DOI: 10.1093/nar/12.11.4507 PMC Full Text View journal details

Analysis Services

A type II restriction endonuclease with an eight nucleotide specificity from Streptomyces fimbriatus.

Contact

WeChat Official Account