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PMID: 6330529 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure and expression of the oncogene c-myc in fresh tumor material from patients with hematopoietic malignancies.

Molecular and cellular biology ·Vol. 4 ·No. 6 ·1984-06-00 ·Pages 1096-103

Rothberg PG, Erisman MD, Diehl RE, Rovigatti UG, Astrin SM

Abstract

c-myc is the cellular gene homologous to the transforming sequence of MC29, an acute avian retrovirus. The human c-myc gene was cloned and used to study the structure and expression of c-myc in a variety of human hematopoietic malignancies. In a careful study of 106 patients, c-myc RNA was found to be expressed at elevated levels in tumor cells of 17 leukemia patients and five lymphoma patients. The c-myc gene was found to be rearranged in two lymphomas, an African Burkitt's lymphoma and a non-Hodgkins lymphoma in leukemic phase. The Burkitt's rearrangement involved the insertion of new DNA sequences upstream from the c-myc 5' coding region, presumably replacing the normal c-myc transcriptional promoter. None of the other 104 patients, including 20 with elevated myc expression, exhibited any evidence of a genetic rearrangement involving the c-myc gene. Our results show that there is a subset of hematopoietic malignancies characterized by elevated expression of c-myc. This elevated expression in most cases is not due to obvious genetic changes (rearrangement, amplification) at the c-myc locus nor to chromosomal translocations in the vicinity of this gene.

MeSH Terms
Base Sequence Burkitt Lymphoma/genetics Cloning, Molecular DNA Restriction Enzymes Humans Leukemia/genetics Lymphoma/genetics Nucleic Acid Hybridization Oncogenes Reference Values
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rothberg P G
Erisman M D
Diehl R E
Rovigatti U G
Astrin S M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.6.1096-1103.1984
Published
1984-06-00
Pages
1096-103
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368878
Subset
IM
Grants
NCI NIH HHS · CA 27797 · United States
NCI NIH HHS · CA-09035 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.6.1096-1103.1984 PMC Full Text View journal details
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