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PMID: 6328511 Published · ppublish English Journal Article

Biologically active protease of foot and mouth disease virus is expressed from cloned viral cDNA in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 11 ·1984-06-00 ·Pages 3351-5

Klump W, Marquardt O, Hofschneider PH

Abstract

Foot and mouth disease virus O1K cDNA had been cloned in Escherichia coli. Here we report on in vitro recombination of cDNA fragments according to the cDNA restriction map and on expression of viral proteins in E. coli. Use was made of the expression vector pPLVP1 , which is known to express the virus capsid protein VP1. Recombined cDNAs of various sizes were inserted downstream from the VP1 gene. The constructed plasmids differ from each other in the number of virus genes coding for nonstructural proteins. The effects caused by their expression in E. coli are compared. It is shown that the virus protease is expressed in E. coli as an active enzyme that recognizes the simultaneously produced virus-specific polyprotein as substrate. The virus protease gene was mapped 5'-adjacent to the virus replicase gene.

MeSH Terms
Aphthovirus/enzymology,genetics Chromosome Mapping Cloning, Molecular Escherichia coli/genetics Gene Expression Regulation Molecular Weight Peptide Hydrolases/genetics Substrate Specificity
Chemicals
Peptide Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Klump W
Marquardt O
Hofschneider P H
References (31)
31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.11.3351
Published
1984-06-00
Pages
3351-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345505
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.11.3351 PMC Full Text View journal details
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