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PMID: 6325174 Published · ppublish English Journal Article

Biosynthesis of the epidermal growth factor receptor in A431 cells.

The EMBO journal ·Vol. 3 ·No. 3 ·1984-03-00 ·Pages 531-7

Mayes EL, Waterfield MD

Abstract

A monoclonal antibody R1 against the human epidermal growth factor receptor has been used to study biosynthesis in the carcinoma cell line A431. Two glycoproteins of apparent mol. wts. 95 000 and 160 000 were immunoprecipitated from cells labelled for short times with [35S]methionine or [3H]mannose. Pulse-chase studies show the 160 000 mol. wt. glycoprotein to be a precursor of the 175 000 mol. wt. receptor, but do not establish a precursor role for the 95 000 mol. wt. glycoprotein. Limited proteolysis, peptide mapping, endoglycosidase digestion and the use of monensin and tunicamycin show that the 95 000 mol. wt. glycoprotein is structurally related to the 160 000 mol. wt. glycoprotein and that both glycoproteins have approximately 22 000 - 28 000 mol. wt. of oligosaccharide side chains. Monensin blocks conversion of the 160 000 to the 175 000 mol. wt. mature receptor, a process which involves complexing several of its N-linked oligosaccharide chains. Pulse-chase studies showed that an immunoprecipitable polypeptide of 115 000 mol. wt., or 95 000 mol. wt., in the presence of monensin, was secreted into the medium at late chase times. The possible mechanisms for the origins of all the receptor-related polypeptides are discussed.

MeSH Terms
Carcinoma Cell Line ErbB Receptors Glycoproteins/biosynthesis Humans Molecular Weight Monensin/pharmacology Peptides/analysis Receptors, Cell Surface/biosynthesis,immunology Tunicamycin/pharmacology
Chemicals
Glycoproteins Peptides Receptors, Cell Surface Tunicamycin Monensin ErbB Receptors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mayes E L
Waterfield M D
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40 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-03-00
Pages
531-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557382
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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