Three new restriction endonucleases MaeI, MaeII and MaeIII from Methanococcus aeolicus.

Nucleic acids research ·Vol. 12 ·No. 6 ·1984-03-26 ·Pages 2619-28

Schmid K, Thomm M, Laminet A, Laue FG, Kessler C, Stetter KO, Schmitt R

Abstract

Three type II restriction endonucleases, MaeI, MaeII and MaeIII, with novel site specificities have been isolated and purified from the archaebacterium Methanococcus aeolicus PL-15/H. The recognition sequences of these enzymes are (formula: see text) with the sites of cleavage as indicated by the arrows. The sequences were confirmed by restriction and computer analyses on sequenced DNA's of plasmid pBR322, bacteriophages lambda and phi X174 and virus SV40.

MeSH Terms

Base Sequence DNA Restriction Enzymes/isolation & purification,metabolism Deoxyribonucleases, Type II Site-Specific Euryarchaeota/enzymology Kinetics Substrate Specificity

Chemicals

DNA Restriction Enzymes endodeoxyribonuclease MaeII endodeoxyribonuclease MaeIII CTAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Schmid K

Thomm M

Laminet A

Laue F G

Kessler C

Stetter K O

Schmitt R

References (14)

14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.6.2619

Published

1984-03-26

Pages

2619-28

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318694

Subset

External Links

PubMed source DOI: 10.1093/nar/12.6.2619 PMC Full Text View journal details

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Three new restriction endonucleases MaeI, MaeII and MaeIII from Methanococcus aeolicus.

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