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PMID: 6324073 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A functional component of the sea urchin H2A gene modulator contains an extended sequence homology to a viral enhancer.

Nucleic acids research ·Vol. 11 ·No. 23 ·1983-12-10 ·Pages 8123-36

Grosschedl R, Mächler M, Rohrer U, Birnstiel ML

Abstract

The DNA sequences imparting a maximal rate of sea urchin H2A gene transcription in the frog oocyte nucleus were narrowed down by deletion mapping to a DNA segment -165 to -111, far-upstream of the H2A mRNA cap site. C to T base changes in this area create strong down mutations, hence the primary structure of this DNA sequence is of paramount importance to the H2A gene expression. Sequence comparisons suggest that the -165 to -111 region may contain two essential sequence blocks. Most strikingly, the -135 area contains a 14 out of 17 basepair homology to the Moloney murine sarcoma virus enhancer and to topologically related 5' LTR-sequences of the simian sarcoma virus and the murine Friend spleen focus forming virus.

MeSH Terms
Animals Base Sequence Cell Nucleus/metabolism DNA Restriction Enzymes Female Genes Histones/genetics Mutation Oocytes/metabolism Plasmids RNA, Messenger/genetics Sea Urchins Transcription, Genetic Xenopus
Chemicals
Histones RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grosschedl R
Mächler M
Rohrer U
Birnstiel M L
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40 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.23.8123
Published
1983-12-10
Pages
8123-36
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326570
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.23.8123 PMC Full Text View journal details
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