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PMID: 6323919 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role of 2-ketobutyrate as an alarmone in E. coli K12: inhibition of adenylate cyclase activity mediated by the phosphoenolpyruvate: glycose phosphotransferase transport system.

Molecular & general genetics : MGG ·Vol. 193 ·No. 3 ·1984-00-00 ·Pages 467-72

Daniel J, Joseph E, Danchin A

Abstract

2-ketobutyrate and its analogues were found to inhibit strongly and transiently the rate of beta-galactosidase synthesis in Escherichia coli K12. This effect was ascribed to a strong and transient inhibition of the adenylate cyclase activity. By using pts mutants, we showed, in agreement with our previous results (Daniel et al. 1983), that the likely target of 2-ketobutyrate and its analogues is the phosphoenolpyruvate: glycose phosphotransferase transport system (PTS). Furthermore, evidence for such a cascade effect caused by 2-ketobutyrate and its analogues allowed us to corroborate our previous proposal (Daniel et al. 1983) that 2-ketobutyrate, a precursor of isoleucine, acts as an E. coli alarmone monitoring the passage from anaerobic to aerobic growth conditions.

MeSH Terms
Adenylyl Cyclase Inhibitors Butyrates/toxicity Cyclic AMP/metabolism Escherichia coli/drug effects,enzymology,genetics Galactosidases/genetics Genotype Kinetics Phosphoenolpyruvate Sugar Phosphotransferase System/genetics,metabolism Species Specificity Structure-Activity Relationship beta-Galactosidase/genetics
Chemicals
Adenylyl Cyclase Inhibitors Butyrates alpha-ketobutyric acid Cyclic AMP Phosphoenolpyruvate Sugar Phosphotransferase System phosphoenolpyruvate-glucose phosphotransferase Galactosidases beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Daniel J
Joseph E
Danchin A
References (15)
15 references, click to expand
  1. 2-Ketobutyrate: a putative alarmone of Escherichia coli.
    Mol Gen Genet. 1983;190(3):452-8 PMID: 6348482
  2. Isoleucine and valine metabolism in Escherichia coli. VII. A negative feedback mechanism controlling isoleucine biosynthesis.
    J Biol Chem. 1958 Aug;233(2):415-20 PMID: 13563512
  3. A rapid test for the rel A mutation in E. coli.
    Biochem Biophys Res Commun. 1976 Apr 5;69(3):751-8 PMID: 773380
  4. Catabolite repression in Escherichia coli mutants lacking cyclic AMP receptor protein.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5799-801 PMID: 6255466
  5. Guanosine 5'-diphosphate 3'-diphosphate (ppGpp): positive effector for histidine operon transcription and general signal for amino-acid deficiency.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4389-93 PMID: 1105582
  6. Carbohydrate transport in bacteria.
    Microbiol Rev. 1980 Sep;44(3):385-418 PMID: 6999324
  7. Correlation between the serine sensitivity and the derepressibility of the ilv genes in Escherichia coli relA- mutants.
    Mol Gen Genet. 1978 Sep 20;165(1):21-30 PMID: 362163
  8. The phosphoenolpyruvate-dependent carbohydrate: phosphotransferase system enzymes II as chemoreceptors in chemotaxis of Escherichia coli K 12.
    Mol Gen Genet. 1981;183(1):163-70 PMID: 7035817
  9. THE INFLUENCE OF NITRATE AND NITRITE REDUCTION ON CATABOLITE REPRESSION IN ESCHERICHIA COLI.
    Biochim Biophys Acta. 1965 May 4;100:553-66 PMID: 14347951
  10. Genetics and function of DNA ligase in Escherichia coli.
    J Mol Biol. 1973 Jul 15;77(4):531-47 PMID: 4353284
  11. Catabolite repression.
    Cold Spring Harb Symp Quant Biol. 1961;26:249-56 PMID: 14468226
  12. Multiple regulation of the activity of adenylate cyclase in Escherichia coli.
    Mol Gen Genet. 1982;185(2):262-8 PMID: 6283317
  13. Regulation of sugar transport in Salmonella typhimurium.
    Ann Microbiol (Paris). 1982 Mar-Apr;133(2):261-7 PMID: 7044216
  14. Interaction of enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system with adenylate cyclase of Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):2920-4 PMID: 1103128
  15. Vinylglycolic acid. An inactivator of the phosphoenolpyruvate-phosphate transferase system in Escherichia coli.
    J Biol Chem. 1973 Aug 10;248(15):5456-62 PMID: 4588683
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00382085
Published
1984-00-00
Pages
467-72
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00382085
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