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PMID: 6322111 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the dsRNA genomic segment 7 of Simian 11 rotavirus.

Nucleic acids research ·Vol. 12 ·No. 3 ·1984-02-10 ·Pages 1621-6

Both GW, Bellamy AR, Siegman LJ

Abstract

A full length cDNA copy of dsRNA segment seven of Simian 11 rotavirus has been obtained by standard molecular cloning techniques. Segment seven codes for the non-structural viral protein NCVP4 and is 1104 nucleotides in length with putative 5'- and 3'- terminal non-coding regions of 25 and 134 residues respectively. The longest open reading frame of 315 codons extends from nucleotide 26 to 970 inclusive. However, the presence nearby of two other AUG codons makes it unclear which codon is used for initiation. The second AUG conforms to the Kozak consensus sequence and if utilised, would yield a protein 312 amino acids in length with a nett charge at pH7 of -2.5. Determination of the gene 7 sequence indicates that terminal sequence conservation among rotavirus gene segments is limited to three and two nucleotides at the 5' and 3' ends of the plus strand, respectively.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cell Transformation, Viral Cloning, Molecular DNA/analysis Genes Genes, Viral Haplorhini Plasmids RNA, Double-Stranded/genetics,isolation & purification Rotavirus/genetics Viral Proteins/genetics

Chemicals

RNA, Double-Stranded Viral Proteins DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Both G W

Bellamy A R

Siegman L J

References (10)

10 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/12.3.1621

Published

1984-02-10

Pages

1621-6

Language

English

Region

England

NLM ID

0411011

PMCID

PMC318603

Subset

Databases

GENBANK

X00355

External Links

PubMed source DOI: 10.1093/nar/12.3.1621 PMC Full Text View journal details

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Nucleotide sequence of the dsRNA genomic segment 7 of Simian 11 rotavirus.

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