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PMID: 6321771 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Protein processing map of poliovirus.

Journal of virology ·Vol. 49 ·No. 3 ·1984-03-00 ·Pages 873-80

Pallansch MA, Kew OM, Semler BL, Omilianowski DR, Anderson CW, Wimmer E, Rueckert RR

Abstract

Five previously unmapped proteins (5a, 7d, 8, 9b, and 10) were located on the proteolytic processing map of the polyprotein. One of the proteins, 9b, appears to be the sister fragment of a cleavage reaction (P3-9 leads to P3-9b + VPg). Two of the other newly mapped proteins, 8 and 10, have been identified as sister fragments of X-related proteins 3b and 5b; thus, P2-3b leads to P2-8 + P2-5b and P2-5b leads to P2-10 + P2-X. The remaining proteins, 5a and 7d, mapped in the 1b protein and appear to result from the cleavages P3-1b leads to P3-5a + P3-6b and P3-4b leads to P3-7d + P3-6b. These assignments account for over 95% of the total polioviral proteins and complete the mapping of the major processing pathways.

MeSH Terms
Amino Acid Sequence HeLa Cells Humans Models, Chemical Poliovirus/metabolism Protein Processing, Post-Translational Trypsin/metabolism Viral Proteins/analysis
Chemicals
Viral Proteins Trypsin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pallansch M A
Kew O M
Semler B L
Omilianowski D R
Anderson C W
Wimmer E
Rueckert R R
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35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.49.3.873-880.1984
Published
1984-03-00
Pages
873-80
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255549
Subset
IM
Grants
NIAID NIH HHS · AI 05935 · United States
NIAID NIH HHS · AI 15122 · United States
NCI NIH HHS · CA 28146 · United States
External Links
PubMed source DOI: 10.1128/JVI.49.3.873-880.1984 PMC Full Text View journal details
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