Home LiteratureArticle Details
PMID: 215997 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell-free synthesis and processing of the proteins of poliovirus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 12 ·1978-12-00 ·Pages 5807-11

Shih DS, Shih CT, Kew O, Pallansch M, Rueckert R, Kaesberg P

Abstract

Poliovirus RNA can be translated completely and accurately in rabbit reticulocyte lysates; the nascent poly-protein is processed to give primary products 1a, X, and 1b indistinguishable from those made in poliovirus-infected HeLa cells. The capsid precursor protein 1a is processed to form the capsid proteins VP0, VP1, and VP3, while the noncapsid precursor 1b is processed to form protein 2.

MeSH Terms
Animals Cell-Free System Kinetics Molecular Weight Peptide Fragments/analysis Peptide Hydrolases/metabolism Poliovirus/metabolism Protein Precursors/biosynthesis Rabbits Reticulocytes Viral Proteins/biosynthesis
Chemicals
Peptide Fragments Protein Precursors Viral Proteins Peptide Hydrolases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Shih D S
Shih C T
Kew O
Pallansch M
Rueckert R
Kaesberg P
References (21)
21 references, click to expand
  1. Identification of a polypeptide encoded by the avian sarcoma virus src gene.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1567-71 PMID: 206909
  2. Cell-free hemoglobin synthesis. II. Characteristics of the transfer ribonucleic acid-dependent assay system.
    J Biol Chem. 1970 May 10;245(9):2342-9 PMID: 4909890
  3. Proteins of Rous-associated virus 61, an avian retrovirus: common precursor for glycoproteins gp85 and gp35 and use of pactamycin to map translational order of proteins in the gag, pol, and env genes.
    J Virol. 1978 May;26(2):380-8 PMID: 77910
  4. Cell-free synthesis of Rauscher murine leukemia virus "gag" and "gag-pol" precursor polyproteins from virion 35 S RNA in a mRNA-dependent translation system derived from mouse tissue culture cells.
    Virology. 1978 May 15;86(2):329-43 PMID: 78568
  5. Translation of encephalomyocarditis virus RNA in vitro yields an active proteolytic processing enzyme.
    Eur J Biochem. 1978 Apr 17;85(2):457-62 PMID: 206439
  6. Complete translation of encephalomyocarditis virus RNA and faithful cleavage of virus-specific proteins in a cell-free system from Krebs-2 cells.
    FEBS Lett. 1978 Mar 1;87(1):7-11 PMID: 204520
  7. Inhibition of translation by poliovirus: inactivation of a specific initiation factor.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2732-6 PMID: 208073
  8. Picornaviral capsid assembly: similarity of rhinovirus and enterovirus precursor subunits.
    J Virol. 1977 Feb;21(2):548-53 PMID: 189086
  9. HeLa cell poly(A)- mRNA codes for a subset of poly(A)+ mRNA-directed proteins with an actin as a major product.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4801-5 PMID: 270712
  10. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  11. Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis.
    Anal Biochem. 1974 Jun;59(2):599-609 PMID: 4134777
  12. On the structure of rhinovirus 1A.
    Virology. 1971 May;44(2):259-70 PMID: 4327717
  13. Translation of reovirus mRNA, poliovirus RNA and bacteriophage Qbeta RNA in cell-free extracts of mammalian cells.
    Methods Enzymol. 1974;30:709-23 PMID: 4369395
  14. An automatic high-precision acrylamide gel fractionator.
    Anal Biochem. 1972 Jun;47(2):321-8 PMID: 4338614
  15. Virus-specific proteins synthesized in encephalomyocarditis virus-infected HeLa cells.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3083-7 PMID: 4332006
  16. A comparison of the virus-specific polypeptides of encephalomyocarditis virus, human rhinovirus-1A, and poliovirus.
    Virology. 1973 Dec;56(2):439-53 PMID: 4357051
  17. Morphogenesis of poliovirus. I. Association of the viral RNA with coat protein.
    J Mol Biol. 1968 Apr 28;33(2):369-78 PMID: 4302632
  18. Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1799-803 PMID: 4515941
  19. Mechanism of premature polypetide termination in a mouse ascites cell-free system programmed by encephalomyocarditis viral RNA.
    J Virol. 1976 May;18(2):788-92 PMID: 178909
  20. Complete translation of poliovirus RNA in a eukaryotic cell-free system.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):4157-61 PMID: 172898
  21. 3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4257-61 PMID: 81487
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.12.5807
Published
1978-12-00
Pages
5807-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393064
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.12.5807 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2