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PMID: 6317657 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transposon mutagenesis of Pseudomonas aeruginosa exoprotease genes.

Journal of bacteriology ·Vol. 157 ·No. 1 ·1984-01-00 ·Pages 7-12

Stapleton MJ, Jagger KS, Warren RL

Abstract

Transposon Tn5 was used to generate protease-deficient insertion mutants of Pseudomonas aeruginosa. The presence of Tn5 in the chromosome of P. aeruginosa was demonstrated by transduction and DNA-DNA hybridization. The altered protease production and kanamycin resistance were cotransduced into a wild-type P. aeruginosa strain. A radiolabeled probe of Tn5 DNA hybridized to specific BamHI fragments isolated from the insertion mutants. Two independently isolated Tn5 insertion mutants had reduced protease production, partially impaired elastase activity, and no immunologically reactive alkaline protease.

MeSH Terms

Conjugation, Genetic DNA Transposable Elements DNA, Bacterial/genetics Exopeptidases Genes, Bacterial Mutation Nucleic Acid Hybridization Peptide Hydrolases/genetics Pseudomonas aeruginosa/enzymology,genetics Transduction, Genetic

Chemicals

DNA Transposable Elements DNA, Bacterial Exopeptidases Peptide Hydrolases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Stapleton M J

Jagger K S

Warren R L

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28 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.157.1.7-12.1984

Published

1984-01-00

Pages

7-12

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215121

Subset

External Links

PubMed source DOI: 10.1128/jb.157.1.7-12.1984 PMC Full Text View journal details

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Transposon mutagenesis of Pseudomonas aeruginosa exoprotease genes.

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