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PMID: 6315197 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Antibody-dependent cell-mediated cytotoxicity and spontaneous cell-mediated cytotoxicity against cells infected with porcine transmissible gastroenteritis virus.

Canadian journal of comparative medicine : Revue canadienne de medecine comparee ·Vol. 47 ·No. 3 ·1983-07-00 ·Pages 298-303

Cepica A, Derbyshire JB

Abstract

The objective of this study was to determine whether porcine peripheral blood leukocytes and intestinal intraepithelial leukocytes can mediate antibody-dependent cell-mediated cytotoxicity and spontaneous cell-mediated cytotoxicity against target cells infected with transmissible gastroenteritis virus. Peripheral blood leukocytes collected from six young adult pigs and intraepithelial leukocytes from a further five pigs were used as effector cells in chromium release assays against PK-15 cells persistently infected with transmissible gastroenteritis virus. Both peripheral blood leukocytes and intraepithelial leukocytes were capable of mediating antibody-dependent cell-mediated cytotoxicity and spontaneous cell-mediated cytotoxicity against PK-15 transmissible gastroenteritis cells. While the peripheral blood leukocytes mediated lower levels of specific 51Cr release in spontaneous cell-mediated cytotoxicity than in antibody-dependent cell-mediated cytotoxicity, the intraepithelial leukocytes were more effective in spontaneous cell-mediated cytotoxicity than antibody-dependent cell-mediated cytotoxicity.

MeSH Terms

Animals Antibody-Dependent Cell Cytotoxicity Cell Line Chromium Radioisotopes Coronaviridae/immunology Cytotoxicity Tests, Immunologic/methods Cytotoxicity, Immunologic Epithelial Cells Intestinal Mucosa/cytology Kidney Leukocytes/immunology Swine Transmissible gastroenteritis virus/growth & development,immunology

Chemicals

Chromium Radioisotopes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cepica A

Derbyshire J B

References (20)

20 references, click to expand

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Article Info

Journal

Canadian journal of comparative medicine : Revue canadienne de medecine comparee

Abbr.

Can J Comp Med

ISSN

0008-4050

Published

1983-07-00

Pages

298-303

Language

English

Region

Canada

NLM ID

0151747

PMCID

PMC1235942

Subset

External Links

PubMed source PMC Full Text

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Antibody-dependent cell-mediated cytotoxicity and spontaneous cell-mediated cytotoxicity against cells infected with porcine transmissible gastroenteritis virus.

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