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PMID: 6314259 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Construction of infectious potato spindle tuber viroid cDNA clones.

Nucleic acids research ·Vol. 11 ·No. 19 ·1983-10-11 ·Pages 6821-35

Cress DE, Kiefer MC, Owens RA

Abstract

Contiguous restriction fragments from two cloned partial-length potato spindle tuber viroid (PSTV) cDNAs were used to construct recombinant DNAs containing full-length monomeric and dimeric PSTV cDNA. When five different PSTV cDNA plasmids and RNA isolated from E. coli cells harboring these plasmids were tested for infectivity on tomato, plasmid DNAs containing PSTV cDNA dimers were infectious. RNA transcripts containing the sequence of PSTV from these plasmids were also infectious. The sequences of the viroid progeny and the cloned DNA were identical. In vitro mutagenesis of infectious PSTV cDNAs will allow systematic investigation of the role of specific sequences in viroid replication and pathogenesis.

MeSH Terms
Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes DNA, Recombinant/metabolism DNA, Viral/genetics Escherichia coli/genetics Genes, Viral Plant Viruses/genetics,pathogenicity Plasmids Transcription, Genetic Viroids/genetics
Chemicals
DNA, Recombinant DNA, Viral DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cress D E
Kiefer M C
Owens R A
References (33)
33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.19.6821
Published
1983-10-11
Pages
6821-35
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326416
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.19.6821 PMC Full Text View journal details
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