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PMID: 6314241 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phosphorylation of rat kidney proximal tubular brush border membranes. Role of c-AMP dependent protein phosphorylation in the regulation of phosphate transport.

Pflugers Archiv : European journal of physiology ·Vol. 398 ·No. 3 ·1983-08-00 ·Pages 221-6

Biber J, Malmström K, Scalera V, Murer H

Abstract

A possible correlation between cyclic-AMP dependent protein phosphorylation and altered sodium dependent transport of inorganic phosphate was analyzed in isolated rat renal proximal tubular brush border membrane vesicles. In transiently opened vesicles (opened by an osmotic shock), the addition of gamma-32P-ATP leads to 32P-incorporation into several membrane proteins. The simultaneous addition of cyclic-AMP leads to increased phosphorylation of several proteins (e.g. apparent molecular weights: 40 kD, 46 kD, 55 kD). The addition of ATP, GTP and ITP to the osmotic shock medium leads to an (non-specific) inhibition of the sodium gradient dependent phosphate uptake. No further inhibition of the sodium dependent phosphate transport was observed when membrane vesicles were phosphorylated by ATP in the presence of cyclic-AMP. These data show a lack of correlation between cyclic-AMP dependent protein phosphorylation and altered sodium gradient dependent phosphate transport. Thus, there is no experimental support for the involvement of cyclic-AMP dependent protein phosphorylation as one of the final events in the regulation of phosphate transport across the rat renal proximal tubular brush border membrane.

MeSH Terms

Animals Biological Transport Cyclic AMP/metabolism Cytoplasm/metabolism Glucose/metabolism In Vitro Techniques Kidney Tubules, Proximal/ultrastructure Male Membrane Proteins/metabolism Microvilli/metabolism Phosphates/metabolism Phosphorylation Rats Rats, Inbred Strains Sodium/metabolism

Chemicals

Membrane Proteins Phosphates Sodium Cyclic AMP Glucose

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Biber J

Malmström K

Scalera V

Murer H

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21 references, click to expand

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Article Info

Journal

Pflugers Archiv : European journal of physiology

Abbr.

Pflugers Arch

ISSN

0031-6768

DOI

10.1007/BF00657155

Published

1983-08-00

Pages

221-6

Language

English

Region

Germany

NLM ID

0154720

Subset

External Links

PubMed source DOI: 10.1007/BF00657155 View journal details

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Phosphorylation of rat kidney proximal tubular brush border membranes. Role of c-AMP dependent protein phosphorylation in the regulation of phosphate transport.

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