Effect of parathyrin on the transport properties of isolated renal brush-border vesicles.

The Biochemical journal ·Vol. 172 ·No. 1 ·1978-04-15 ·Pages 49-56

Evers C, Murer H, Kinne R

Abstract

The transport properties of brush-border membrane vesicles isolated by a calcium-precipitation method from the renal cortex of normal and parathyrin (parathyroid hormone)-treated rats were studied by a rapid-filtration technique. Parathyrin elicited a dose-dependent decrease in the Na+-dependent phosphate uptake by the brush-border membrane vesicles, but the uptake of D-glucose, Na+ and mannitol was not affected. A maximum inhibition of 30% was observed after the application of 30 U.S.P. units intramuscularly 1 h before the animals were killed. Intravenous infusion of dibutyryl cyclic AMP (0.5-1.5 MG) also decreased the phosphate uptake by the brush-border vesicles. Both dibutyryl cyclic AMP and parathyrin were ineffective when added in vitro to brush-border membrane vesicles isolated from normal rats. These data suggest that parathyrin exerts its action on the phosphate reabsorption in the renal proximal tubule by affecting the Na+/phosphate co-transport system in the brush-border membrane. The effects of parathyrin on Na+ and glucose transport, however, seem to be due to alterations to the driving forces for transport and not to the brush-border transport systems.

MeSH Terms

Animals Biological Transport/drug effects Cyclic AMP/pharmacology In Vitro Techniques Kidney Cortex/drug effects,enzymology,metabolism Kidney Tubules, Proximal/drug effects,metabolism Kinetics Male Microvilli/metabolism Parathyroid Hormone/pharmacology Phosphates/metabolism Rats Vasopressins/pharmacology

Chemicals

Parathyroid Hormone Phosphates Vasopressins Cyclic AMP

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Evers C

Murer H

Kinne R

References (23)

23 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1720049

Published

1978-04-15

Pages

49-56

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1185660

Subset

External Links

PubMed source DOI: 10.1042/bj1720049 PMC Full Text View journal details

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Effect of parathyrin on the transport properties of isolated renal brush-border vesicles.

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