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PMID: 6313849 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Basolateral potassium channel in turtle colon. Evidence for single-file ion flow.

The Journal of general physiology ·Vol. 82 ·No. 3 ·1983-09-00 ·Pages 297-313

Kirk KL, Dawson DC

Abstract

Treatment of the apical surface of the isolated, ouabain-inhibited turtle colon with the polyene antibiotic amphotericin B permitted the properties of a barium-sensitive potassium conductance in the basolateral membrane to be discerned from the measurements of transepithelial fluxes and electrical currents. Simultaneous measurements of potassium currents and 42K fluxes showed that the movement of potassium was not in accord with simple diffusion. Two other cations, thallium and rubidium, were also permeable and, in addition, exhibited strong interactions with the potassium tracer fluxes. The results indicate that permeant cations exhibit positive coupling, which is consistent with a single-file mechanism of ion translocation through a membrane channel.

MeSH Terms
Animals Biological Transport Cations/metabolism Colon/metabolism Ion Channels/metabolism Ions Models, Biological Polyenes/metabolism Potassium/metabolism Turtles/metabolism
Chemicals
Cations Ion Channels Ions Polyenes Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kirk K L
Dawson D C
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32 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.82.3.297
Published
1983-09-00
Pages
297-313
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2228702
Subset
IM
Grants
NIADDK NIH HHS · AM00994 · United States
NIADDK NIH HHS · AM29786 · United States
External Links
PubMed source DOI: 10.1085/jgp.82.3.297 PMC Full Text View journal details
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