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PMID: 6259361 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ion conductance and ion selectivity of potassium channels in snail neurones.

The Journal of membrane biology ·Vol. 57 ·No. 2 ·1980-12-15 ·Pages 103-18

Reuter H, Stevens CF

Abstract

Delayed potassium channels were studied in internally perfused neurone somata from land snails. Relaxation and fluctuation analysis of this class of ion channels revealed Hodgkin-Huxley type K channels with an average single channel conductance (gamma K) of 2.40 +/- 0.15 pS. The conductance of open channels is independent of voltage and virtually all K channels seem to be open at maximum K conductance (gk) of the membrane. Voltage dependent time constants of activation of gK, calculated from K current relaxation and from cut-off frequencies of power spectra, are very similar indicating dominant first-order kinetics. Ion selectivity of K channels was studied by ion substitution in the external medium and exhibited the following sequence: Tl+ greater than K+ greater than Rb+ greater Cs+ greater than NH4+ greater Li+ greater than Na+. The sequence of the alkali cations does not conform to any of the sequences predicted by Eisenman's theory. However, the data are well accommodated by a new theory assuming a single rate-limiting barrier that governs ion movement through the channel.

MeSH Terms
Animals Cations, Monovalent/metabolism Electric Stimulation Evoked Potentials Helix, Snails Ion Channels/physiology Mathematics Neural Conduction Neurons/physiology Potassium/physiology
Chemicals
Cations, Monovalent Ion Channels Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reuter H
Stevens C F
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32 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01868997
Published
1980-12-15
Pages
103-18
Language
English
Region
United States
NLM ID
0211301
Subset
IM
Grants
NINDS NIH HHS · NS-12961 · United States
External Links
PubMed source DOI: 10.1007/BF01868997 View journal details
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