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PMID: 6312273 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A temporally regulated promoter from Bacillus subtilis is transcribed only by an RNA polymerase with a 37,000 dalton sigma factor.

Molecular & general genetics : MGG ·Vol. 191 ·No. 2 ·1983-00-00 ·Pages 319-25

Goldfarb DS, Wong SL, Kudo T, Doi RH

Abstract

A 1,250 base pair Bacillus subtilis chromosomal HindIII restriction fragment (S fragment) has been cloned into the B. subtilis expression-probe plasmid pGR71. The S fragment induces the expression of the pGR71 chloramphenicol resistance gene shortly after the initiation of sporulation. The transcriptional promoter responsible for the expression of this temporally regulated genetic element has been identified and mapped in vitro. This promoter is recognized exclusively by the minor B. subtilis RNA polymerase that contains the 37,000 dalton sigma factor.

MeSH Terms
Acetyltransferases/genetics Bacillus subtilis/genetics,physiology Chloramphenicol O-Acetyltransferase DNA Restriction Enzymes DNA-Directed RNA Polymerases/genetics Gene Expression Regulation Genes, Bacterial Operon Plasmids Sigma Factor Spores, Bacterial Transcription Factors Transcription, Genetic
Chemicals
Sigma Factor Transcription Factors Acetyltransferases Chloramphenicol O-Acetyltransferase DNA-Directed RNA Polymerases DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Goldfarb D S
Wong S L
Kudo T
Doi R H
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34 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334833
Published
1983-00-00
Pages
319-25
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM19673 · United States
External Links
PubMed source DOI: 10.1007/BF00334833
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