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PMID: 6312076 Published · ppublish English Journal Article

Molecular cloning of the unintegrated squirrel monkey retrovirus genome: organization and distribution of related sequences in primate DNAs.

Journal of virology ·Vol. 47 ·No. 3 ·1983-09-00 ·Pages 434-41

Chiu IM, Andersen PR, Aaronson SA, Tronick SR

Abstract

The closed circular form of the endogenous squirrel monkey type D retrovirus (SMRV) was molecularly cloned in a bacteriophage vector. The restriction map of the biologically active clone was determined and found to be identical to that of the parental SMRV linear DNA except for the deletion of one long terminal repeat. Restriction enzyme analysis and Southern blotting indicated that the SMRV long terminal repeat was approximately 300 base pairs long. The SMRV restriction map was oriented to the viral RNA by using a gene-specific probe from baboon endogenous virus. Restriction enzyme digests of a variety of vertebrate DNAs were analyzed for DNA sequence homology with SMRV by using the cloned SMRV genome as a probe. Consistent with earlier studies, multiple copies of SMRV were detected in squirrel monkey DNA. Related fragments were also detected in the DNAs from other primate species, including humans.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/analysis DNA Restriction Enzymes DNA, Viral/analysis Genes, Viral Humans Nucleic Acid Hybridization Primates/genetics Repetitive Sequences, Nucleic Acid Retroviridae/genetics Saimiri
Chemicals
DNA, Viral DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chiu I M
Andersen P R
Aaronson S A
Tronick S R
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46 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.47.3.434-441.1983
Published
1983-09-00
Pages
434-41
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255284
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.47.3.434-441.1983 PMC Full Text View journal details
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