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PMID: 6311301 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Estimating kinetic constants from single channel data.

Biophysical journal ·Vol. 43 ·No. 2 ·1983-08-00 ·Pages 207-23

Horn R, Lange K

Abstract

The process underlying the opening and closing of ionic channels in biological or artificial lipid membranes can be modeled kinetically as a time-homogeneous Markov chain. The elements of the chain are kinetic states that can be either open or closed. A maximum likelihood procedure is described for estimating the transition rates between these states from single channel data. The method has been implemented for linear kinetic schemes of fewer than six states, and is suitable for nonstationary data in which one or more independent channels are functioning simultaneously. It also provides standard errors for all estimates of rate constants and permits testing of smoothly parameterized subhypotheses of a general model. We have illustrated our approach by analysis of single channel data simulated on a computer and have described a procedure for analysis of experimental data.

MeSH Terms
Animals Humans Ion Channels/metabolism Kinetics Lidocaine/pharmacology Models, Biological Receptors, Cholinergic/metabolism
Chemicals
Ion Channels Receptors, Cholinergic Lidocaine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Horn R
Lange K
References (29)
29 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(83)84341-0
Published
1983-08-00
Pages
207-23
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1329250
Subset
IM
Grants
NICHD NIH HHS · KO4 HD00307 · United States
NINDS NIH HHS · NS 186-08 · United States
NINDS NIH HHS · NS 703-01 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(83)84341-0 PMC Full Text View journal details
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