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PMID: 6311177 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stimulation of prostaglandin production through purinoceptors on cultured porcine endothelial cells.

The Biochemical journal ·Vol. 214 ·No. 1 ·1983-07-15 ·Pages 273-6

Pearson JD, Slakey LL, Gordon JL

Abstract

ATP (approx. 1-300 microM) induces dose-dependent prostacyclin secretion from perfused columns of microcarrier beads with cultured endothelial monolayers attached. The response is transient, shows little tachyphylaxis and can reach approx. 100 times control values. 2-Methylthio-ATP is more potent, ADP slightly less potent, AMP much less potent and adenosine is ineffective. These results are consistent with the presence of a purinoceptor on endothelium linked to the prostacyclin synthetic pathway.

MeSH Terms

Adenine Nucleotides/pharmacology Animals Cells, Cultured Dose-Response Relationship, Drug Endothelium/drug effects,metabolism Epoprostenol/metabolism Prostaglandins/metabolism Receptors, Cell Surface/drug effects Receptors, Purinergic Stimulation, Chemical Swine

Chemicals

Adenine Nucleotides Prostaglandins Receptors, Cell Surface Receptors, Purinergic Epoprostenol

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pearson J D

Slakey L L

Gordon J L

References (13)

13 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2140273

Published

1983-07-15

Pages

273-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1152238

Subset

External Links

PubMed source DOI: 10.1042/bj2140273 PMC Full Text View journal details

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Stimulation of prostaglandin production through purinoceptors on cultured porcine endothelial cells.

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