Home LiteratureArticle Details
PMID: 6258567 Published · ppublish English Journal Article

Metabolism of adenine nucleotides by ectoenzymes of vascular endothelial and smooth-muscle cells in culture.

The Biochemical journal ·Vol. 190 ·No. 2 ·1980-08-15 ·Pages 421-9

Pearson JD, Carleton JS, Gordon JL

Abstract

1. Pig aortic endothelial and smooth-muscle cells in culture rapidly catabolize exogenous ATP, ADP or AMP. 2. In both cell types catabolism is due to Mg2+-stimulated ectoenzymes. 3. Inhibition and substrate-specificity studies suggest that both cell types possess three distinct ectonucleotidases, namely nucleoside triphosphatase (EC 3.6.1.15), nucleoside diphosphatase (EC 3.6.1.6) and 5'-nucleotidase (EC 3.1.3.5), as well as nucleoside diphosphate kinase (EC 2.7.4.6). 4. These ectonucleotidase systems could be of importance in the regulation of neurotransmission, blood platelet function and vasodilation.

MeSH Terms
Acid Anhydride Hydrolases Adenine Nucleotides/metabolism Animals Aorta/enzymology Cells, Cultured Endothelium/enzymology Kinetics Muscle, Smooth, Vascular/enzymology Nucleoside-Diphosphate Kinase/metabolism Nucleoside-Triphosphatase Nucleotidases/metabolism Nucleotides/metabolism Phosphoric Monoester Hydrolases/metabolism Phosphotransferases/metabolism Substrate Specificity Swine
Chemicals
Adenine Nucleotides Nucleotides Phosphotransferases Nucleoside-Diphosphate Kinase Nucleotidases Phosphoric Monoester Hydrolases Acid Anhydride Hydrolases Nucleoside-Triphosphatase nucleoside-diphosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pearson J D
Carleton J S
Gordon J L
References (41)
41 references, click to expand
  1. Characterization of "ecto-ATPase" of human blood platelets.
    Arch Biochem Biophys. 1967 Mar;119(1):173-8 PMID: 4228220
  2. Catabolism of adenine nucleotides by the isolated perfused rat heart.
    Proc Soc Exp Biol Med. 1968 Jan;127(1):33-6 PMID: 5644662
  3. The release of nucleotides, 5-hydroxytryptamine and enzymes from human blood platelets during aggregation.
    J Physiol. 1968 Apr;195(3):715-29 PMID: 5649642
  4. Leukocyte adenosine triphosphatases and the effect of endotoxin on their activity.
    Arch Biochem Biophys. 1968 Jul;126(1):53-8 PMID: 4233581
  5. Disappearance of adenosine diphosphate in vivo.
    J Lab Clin Med. 1969 Jan;73(1):54-9 PMID: 5762164
  6. Metabolism of ( 14 C)adenine and derivatives by cerebral tissues, superfused and electrically stimulated.
    Biochem J. 1972 Feb;126(4):965-73 PMID: 5073246
  7. Purinergic nerves.
    Pharmacol Rev. 1972 Sep;24(3):509-81 PMID: 4404211
  8. Adenylate metabolism and adenosine formation in the heart.
    Am J Physiol. 1972 Nov;223(5):1119-27 PMID: 4654345
  9. The effects of dipyridamole on coronary post-occlusion hyperaemia and on myocardial vasodilatation induced by systemic hypoxia.
    Br J Pharmacol. 1972 Dec;46(4):594-601 PMID: 4655264
  10. Sites of adenosine production in cardiac and skeletal muscle.
    Am J Physiol. 1973 Oct;225(4):938-53 PMID: 4743384
  11. Quantitative thin-layer chromatography of ATP and the products of its degradation in meat tissue.
    J Chromatogr. 1974 Mar 13;90(1):105-11 PMID: 4824651
  12. On the sidedness of plasma membrane enzymes.
    Biochim Biophys Acta. 1974 Apr 29;345(2):180-97 PMID: 4366461
  13. Evidence for ATP action on the cell surface.
    Nature. 1974 Dec 6;252(5483):480-2 PMID: 4279341
  14. Ecto-enzymes of the guinea pig polymorphonuclear leukocyte. I. Evidence for an ecto-adenosine monophosphatase, adenosine triphosphatase, and -p-nitrophenyl phosphates.
    J Biol Chem. 1974 Nov 25;249(22):7111-20 PMID: 4373458
  15. The inhibition of platelet aggregation by an aorta intima extract.
    Thromb Diath Haemorrh. 1974 Dec 31;32(2-3):417-31 PMID: 4450195
  16. Release of ATP from perfused heart during coronary vasodilatation.
    Blood Vessels. 1974;11(3):110-9 PMID: 4451725
  17. 5'-Nucleotidase from smooth muscle of small intestine and from brain. Inhibition of nucleotides.
    Biochemistry. 1975 Jun 3;14(11):2362-6 PMID: 1169962
  18. Uptake of adenosine by dispersed chich embryonic cardiac cells.
    Am J Physiol. 1975 Jan;228(1):62-7 PMID: 167591
  19. The properties and extracellular location of 5'-nucleotidase of the rat fat-cell plasma membrane.
    Biochem J. 1975 Mar;146(3):625-33 PMID: 167725
  20. Effects of adenosine on levels of adenosine cyclic 3',5'-monophosphate in human blood platelets in relation to adenosine incorporation and platelet aggregation.
    Mol Pharmacol. 1975 Sep;11(5):528-44 PMID: 170501
  21. Ecto-5'-nucleotidase of intact cultured C6 rat glioma cells.
    J Biol Chem. 1976 Jul 10;251(13):3900-5 PMID: 819433
  22. A membrane-bound enzyme in rabbit aorta capable of inhibiting adenosine-diphosphate-induced platelet aggregation.
    Lancet. 1977 Aug 13;2(8033):330-2 PMID: 69937
  23. ADPase activity of normal and atherosclerotic human aorta intima.
    Thromb Haemost. 1977 Jun 30;37(3):429-35 PMID: 196356
  24. Pulmonary endothelial cells.
    Fed Proc. 1977 Dec;36(13):2683-91 PMID: 336405
  25. Antithrombotic properties of vascular endothelium.
    Lancet. 1977 Nov 19;2(8047):1075-6 PMID: 72976
  26. Uptake and metabolism of adenosine by pig aortic endothelial and smooth-muscle cells in culture.
    Biochem J. 1978 Feb 15;170(2):265-71 PMID: 637842
  27. Metabolism and uptake of adenosine triphosphate and adenosine by porcine aortic and pulmonary endothelial cells and fibroblasts in culture.
    Circ Res. 1978 Jun;42(6):869-76 PMID: 657449
  28. ADPase activity of isolated perfused rat lung.
    Life Sci. 1978 Apr 24;22(16):1413-20 PMID: 209269
  29. Purification and properties of 5'-nucleotidase from lymphocyte plasma membranes.
    Eur J Biochem. 1978 Jul 3;87(3):459-65 PMID: 28225
  30. Evidence that ADP hydrolysis by human cells is related to thrombogenic potential1.
    Thromb Res. 1978 Aug;13(2):255-66 PMID: 694844
  31. Plasma membrane adenosine triphosphatases in rat peritoneal mast cells and macrophages--the relation of the mast cell enzyme to histamine release.
    Biochem Pharmacol. 1978;27(11):1561-9 PMID: 81056
  32. Mg2+-ATPase as a membrane ecto-enzyme of human granulocytes. Inhibitors, activators and response to phagocytosis.
    Biochim Biophys Acta. 1978 Oct 4;512(3):525-38 PMID: 152129
  33. Comparative degradation of adenylnucleotides by cultured endothelial cells and fibroblasts.
    Biochem Biophys Res Commun. 1978 Nov 14;85(1):183-9 PMID: 743274
  34. ATP induces nucleotide permeability in rat mast cells.
    Nature. 1979 Jun 7;279(5713):541-2 PMID: 450099
  35. Enzyme activities in endothelial cells and smooth muscle cells from swine aorta.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2532-5 PMID: 223146
  36. ADP metabolism in vascular tissue, a possible thrombo-regulatory mechanism.
    Thromb Res. 1979;14(6):901-14 PMID: 473125
  37. Vascular endothelial and smooth muscle cells in culture selectively release adenine nucleotides.
    Nature. 1979 Oct 4;281(5730):384-6 PMID: 481603
  38. Radioimmunoassay of 6-oxoprostaglandin F1 alpha and prostaglandin E2 produced by pig aortic endothelium in culture [proceedings].
    Biochem Soc Trans. 1979 Oct;7(5):1065-6 PMID: 510701
  39. Effects of coronary vasodilator drugs on the uptake and release of adenosine in cardiac cells.
    Biochem Pharmacol. 1979 Sep 1;28(17):2617-24 PMID: 518672
  40. Plaque formation and isolation of pure lines with poliomyelitis viruses.
    J Exp Med. 1954 Feb;99(2):167-82 PMID: 13130792
  41. Plaque production by the polyoma virus.
    Virology. 1959 Jul;8(3):396-7 PMID: 13669362
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1900421
Published
1980-08-15
Pages
421-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162107
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1900421 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2