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PMID: 5649642 Published · ppublish English Journal Article

The release of nucleotides, 5-hydroxytryptamine and enzymes from human blood platelets during aggregation.

The Journal of physiology ·Vol. 195 ·No. 3 ·1968-04-00 ·Pages 715-29

Mills DC, Robb IA, Roberts GC

Abstract

1. Adenosine diphosphate (ADP) and adrenaline caused the aggregation of human platelets suspended in plasma containing citrate anticoagulant and stirred at 37 degrees C. The aggregation occurred in two phases and the second phase was associated with the appearance in the plasma of up to 30% of the ATP and 55% of the ADP present in the platelets. The concentration of ADP appearing in the plasma was up to 7 times the concentration added.2. Radioactivity was released by ADP and by adrenaline from platelets labelled with radioactive 5-hydroxytryptamine; this release was closely correlated with the second phase of aggregation and with the release of nucleotides.3. Acid phosphatase, beta-glucuronidase and adenylate kinase were released to a small extent during second phase aggregation by ADP or adrenaline; thrombin and collagen particles caused significantly greater release of beta-glucuronidase than of either acid phosphatase or of adenylate kinase.4. Morphological changes indicating degranulation of the platelets were observed during the second phase of aggregation produced by adrenaline and by ADP.5. The second phase of aggregation, degranulation of platelets, and the release of nucleotides, of labelled 5-hydroxytryptamine and of enzymes, were all inhibited by concentrations of amitriptyline which did not inhibit aggregation.

MeSH Terms
Acid Phosphatase/metabolism Adenine Nucleotides/blood,pharmacology Adenosine Triphosphate/blood Amitriptyline/pharmacology Blood Coagulation/physiology Blood Platelets/enzymology,metabolism,pathology Carbon Isotopes Collagen/pharmacology Enzymes/metabolism Epinephrine/pharmacology Glucuronidase/metabolism Humans Imipramine/pharmacology In Vitro Techniques Microscopy, Electron Nucleotides/metabolism Phosphotransferases/metabolism Serotonin/metabolism Thrombin/pharmacology
Chemicals
Adenine Nucleotides Carbon Isotopes Enzymes Nucleotides Amitriptyline Serotonin Adenosine Triphosphate Collagen Phosphotransferases Acid Phosphatase Glucuronidase Thrombin Imipramine Epinephrine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mills D C
Robb I A
Roberts G C
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33 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1968.sp008484
Published
1968-04-00
Pages
715-29
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1351696
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1968.sp008484 PMC Full Text View journal details
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