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PMID: 6309673 Published · ppublish English Comparative Study Journal Article

Complexes of hepatitis B surface antigen and immunoglobulin M in the sera of patients with hepatitis B virus infection.

Infection and immunity ·Vol. 41 ·No. 3 ·1983-09-00 ·Pages 950-8

Palla M, Rizzi R, Toti M, Almi P, Rizzetto M, Bonino F, Purcell R

Abstract

Hepatitis B surface antigen (HBsAg) bound to immunoglobulin M (IgM) was detected in sera of HBsAg carriers by a radioimmunoassay based on selective absorption of the immunoglobulin on a solid phase coated with antiserum to human IgM. Isopycnic banding and rate-zonal sedimentation have shown that the reaction is related to particulate forms of the HBsAg complexed with IgM. The binding of IgM possibly occurred because of a selective affinity of these molecules to the surface of HBsAg particles. HBsAg/IgM was found transiently in 24 of 25 (96%) patients with acute self-limited hepatitis B and persistently in 6 of 25 patients whose acute hepatitis B progressed to chronicity. It was also found in 20 of 39 (51%) chronic HBsAg carriers with inactive and asymptomatic infection. The HBsAg/IgM phenomenon is not dependent on replication of hepatitis B virions; its persistence in patients with acute hepatitis B may provide complementary evidence of transition of the infection to chronicity.

MeSH Terms
Animals Antibodies, Viral/analysis Antigen-Antibody Complex/analysis Carrier State/immunology Hepatitis A/immunology Hepatitis B/immunology Hepatitis B Antibodies/analysis Hepatitis B Core Antigens/analysis Hepatitis B Surface Antigens/analysis Hepatitis B e Antigens/analysis Hepatitis C/immunology Hepatovirus/immunology Humans Immunoglobulin M/analysis Pan troglodytes
Chemicals
Antibodies, Viral Antigen-Antibody Complex Hepatitis B Antibodies Hepatitis B Core Antigens Hepatitis B Surface Antigens Hepatitis B e Antigens Immunoglobulin M
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Palla M
Rizzi R
Toti M
Almi P
Rizzetto M
Bonino F
Purcell R
References (37)
37 references, click to expand
  1. Presence of receptors for polymerized albumin in HBsAg-containing hepatocytes and hepatoma cell line.
    Hepatology. 1981 Mar-Apr;1(2):132-6 PMID: 6269978
  2. A receptor for polymerized human and chimpanzee albumins on hepatitis B virus particles co-occurring with HBeAg.
    Gastroenterology. 1979 Feb;76(2):242-7 PMID: 103774
  3. Antigenic sites related to human serum proteins in HBsAg: isolation from normal human serum of a high-molecular-weight glycoprotein reacting with antialbumin.
    J Med Virol. 1978;2(3):231-45 PMID: 81269
  4. Sites that bind polymerized albumin on hepatitis B surface antigen particles: detection by radioimmunoassay.
    Infect Immun. 1979 Oct;26(1):125-30 PMID: 500199
  5. HBsAg immune complexes in the course of infection with hepatitis B virus.
    Clin Exp Immunol. 1979 Jun;36(3):371-8 PMID: 487641
  6. Detection of circulating immune complexes in hepatitis by means of a new method employing 125i-antibody. circulating immune complexes in hepatitis.
    Acta Hepatogastroenterol (Stuttg). 1978 Jun;25(3):185-8 PMID: 665119
  7. Australia antigen-antibody complexes.
    Nature. 1970 Apr 4;226(5240):83-4 PMID: 4985010
  8. Cryoglobulinemia--a study of twenty-nine patients. I. IgG and IgM cryoglobulins and factors affecting cryoprecipitability.
    Am J Med. 1966 Jun;40(6):828-36 PMID: 4956870
  9. Transmission of the hepatitis B virus-associated delta antigen to chimpanzees.
    J Infect Dis. 1980 May;141(5):590-602 PMID: 6989929
  10. Enzyme-linked immunosorbent assay for detection of immunoglobulin M antibody to hepatitis B core antigen.
    J Clin Microbiol. 1981 Mar;13(3):405-9 PMID: 7240384
  11. Subtyping of hepatitis-associated antigen (HB-Ag); simplified technique with counterelectrophoresis.
    J Immunol. 1972 Sep;109(3):420-5 PMID: 4115539
  12. Detection and quantitation of hepatitis-B surface antigen immune complexes (HBsAg-ICs) by an antigen-specific method. I. Detection and quantitation of in vitro prepared HBsAg-ICs.
    J Immunol Methods. 1980;33(3):293-300 PMID: 6154746
  13. Radioimmunoassay for albumin-binding sites associated with HBsAg: correlation of results with the presence of e-antigen in serum.
    Intervirology. 1979;11(2):128-32 PMID: 429144
  14. Hepatitis B circulating immune complexes. Characterization by radioimmunoprecipitation--PEG assay (RIPEGA).
    J Immunol Methods. 1977;15(3):201-9 PMID: 853223
  15. Antigen-specific detection of HBsAG-containing immune complexes in the course of hepatitis B virus infection.
    Clin Exp Immunol. 1979 Aug;37(2):376-80 PMID: 91465
  16. Serum IgM and IgG responses in postnatally acquired rubella.
    Lancet. 1969 Jul 12;2(7611):65-8 PMID: 4182759
  17. Detection of hepatitis B antigen in circulating immune complexes in acute and chronic hepatitis.
    Scand J Immunol. 1977;6(12):1297-304 PMID: 24265
  18. Australia antigen immune complexes in patients with different forms of hepatitis.
    J Infect Dis. 1971 Mar;123(3):251-6 PMID: 5000074
  19. Preparation of iodine-131 labelled human growth hormone of high specific activity.
    Nature. 1962 May 5;194:495-6 PMID: 14450081
  20. Interactions between polymerized human albumin, hepatitis B surface antigen, and complement: II. Involvement of Clq in or near the hepatitis B surface antigen receptor for polyalbumin.
    J Med Virol. 1981;7(3):193-204 PMID: 6270277
  21. Detection of a new antibody system reacting with Dane particles in hepatitis B virus infection.
    Br Med J. 1978 Oct 14;2(6144):1056-8 PMID: 81702
  22. Interaction of hepatitis B surface antigen with polymerized human serum albumin.
    J Med Virol. 1979;4(3):177-85 PMID: 536742
  23. Persistence of circulating HBsAg/IgM complexes in acute viral hepatitis, type B: an early marker of chronic evolution.
    Lancet. 1982 Aug 14;2(8294):358-60 PMID: 6124761
  24. Serodiagnosis of viral hepatitis A by a solid-phase radioimmunoassay specific for IgM antibodies.
    Scand J Infect Dis. 1981;13(1):5-9 PMID: 6264589
  25. HBsAg-associated albumin receptors and antialbumin antibodies in sera of patients with liver disease.
    Gastroenterology. 1981 Feb;80(2):260-4 PMID: 6161062
  26. A new principle for the detection of specific IgM antibodies applied in an ELISA for hepatitis A.
    J Med Virol. 1979;4(1):25-32 PMID: 231096
  27. Specificities of albumin receptors and albumin antibodies.
    Infect Immun. 1981 Jun;32(3):1292-4 PMID: 7251167
  28. Interactions between polymerized human albumin, hepatitis B surface antigen, and complement: I. Binding of polyalbumin to Clq.
    J Med Virol. 1981;7(3):181-92 PMID: 6169799
  29. Circulating immune complexes in the serum in systemic lupus erythematosus and in carriers of hepatitis B antigen. Quantitation by binding to radiolabeled C1q.
    J Clin Invest. 1974 Aug;54(2):297-309 PMID: 4847246
  30. Radioimmunoassay for the detection of the core of the Dane particle and antibody to it.
    Intervirology. 1974;2(4):231-43 PMID: 4854012
  31. A rountine diagnostic test for IgA and IgM antibodies to rubella virus: absorption of IgG with Staphylococcus aureus.
    J Infect Dis. 1974 Sep;130(3):268-73 PMID: 4411735
  32. Immune complexes in hepatitis B antigen-associated periarteritis nodosa. Detection by antibody-dependent cell-mediated cytotoxicity and the Raji cell assay.
    Am J Med. 1977 May;62(5):783-91 PMID: 16490
  33. The hepatitis B virus-associated delta antigen: isolation from liver, development of solid-phase radioimmunoassays for delta antigen and anti-delta and partial characterization of delta antigen.
    J Immunol. 1980 Jul;125(1):318-24 PMID: 6769999
  34. IgM antibody to hepatitis B core antigen as a diagnostic parameter of acute infection with hepatitis B virus.
    J Infect Dis. 1981 Jun;143(6):803-9 PMID: 6788859
  35. Immune complexes in hepatitis.
    Lancet. 1969 Nov 8;2(7628):983-6 PMID: 4186979
  36. Relationship of large hepatitis B surface antigen polypeptide to human serum albumin.
    Infect Immun. 1980 May;28(2):459-63 PMID: 7399672
  37. IgM levels and IgM-mediated immune responses in patients with acute hepatitis A, acute hepatitis B and chronic HB antigenaemia.
    Clin Exp Immunol. 1976 Jan;23(1):69-72 PMID: 1261090
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.41.3.950-958.1983
Published
1983-09-00
Pages
950-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC264593
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.41.3.950-958.1983 PMC Full Text View journal details
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