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PMID: 6308627 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Efficient generation in vitro, from human peripheral blood cells, of monoclonal Epstein-Barr virus transformants producing specific antibody to a variety of antigens without prior deliberate immunization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 14 ·1983-07-00 ·Pages 4484-8

Winger L, Winger C, Shastry P, Russell A, Longenecker M

Abstract

This paper describes a simple protocol for the efficient generation of large numbers of human monoclonal antibody-producing cells. This system is based on initial limiting-dilution culture after Epstein-Barr virus exposure of highly enriched precursors selected from peripheral blood mononuclear cells. Precursors can be enriched by using rosetting or panning approaches. Antibodies to erythrocytes, a mouse mammary carcinoma, DNA, and sperm antigens, produced without any deliberate immunization, are described. Large-scale human monoclonal antibody production may be facilitated by a combination of this protocol with a human cellular fusion system. For efficient precursor analysis and short-term (2 months or more) monoclonal antibody production, however, the system described here may be sufficient.

MeSH Terms

Animals Antibodies, Viral/immunology Antigen-Antibody Complex Antigens, Viral/immunology Cell Transformation, Viral Cells, Cultured Chickens DNA/immunology Fluorescent Antibody Technique Genotype Hemagglutination Tests Herpesvirus 4, Human/immunology Humans Male Monocytes/immunology Radioimmunoassay Rosette Formation Sea Urchins Spermatozoa/immunology

Chemicals

Antibodies, Viral Antigen-Antibody Complex Antigens, Viral DNA

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Winger L

Winger C

Shastry P

Russell A

Longenecker M

References (32)

32 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.80.14.4484

Published

1983-07-00

Pages

4484-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC384063

Subset

External Links

PubMed source DOI: 10.1073/pnas.80.14.4484 PMC Full Text View journal details

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Efficient generation in vitro, from human peripheral blood cells, of monoclonal Epstein-Barr virus transformants producing specific antibody to a variety of antigens without prior deliberate immunization.

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